SNAP23促进卵巢癌的恶性进程。
SNAP23 promotes the malignant process of ovarian cancer.
作者信息
Sun Qi, Huang Xing, Zhang Quanli, Qu Junwei, Shen Yang, Wang Xin, Sun Haijun, Wang Jie, Xu Lin, Chen Xiaoxiang, Ren Binhui
机构信息
Department of Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu, China, 210009.
Department of Cardiothoracic Surgery, Jinling Hospital, Southern Medical University, East Zhongshan Road 305, Xuanwu District, Nanjing, Jiangsu, 210002, People's Republic of China.
出版信息
J Ovarian Res. 2016 Nov 17;9(1):80. doi: 10.1186/s13048-016-0289-9.
BACKGROUND
Ovarian cancer (OC) was the primary malignant gynecological cancer and SNARE protein is closely related with tumor progression. Here, we identified SNAP23, a member of SNARE complex, as a potential oncogene in OC.
METHODS
We determined the expression of SNAP23 in OC tissues and explored the clinical significance through bioinformatics analysis. The effects of SNAP23 on OC cell proliferation, migration, invasion, cell cycle and apoptosis were then evaluated in vitro.
RESULTS
SNAP23 is hyper-expressed in OC tumor tissues compared to normal tissues, and increased expression of SNAP23 is associated with a poor progression free survival (HR = 1.24, 95% CI = 1.07-1.44, p = 0.0042). SNAP23 knock down increases cell apoptosis and inhibits cell proliferation, migration and invasion of OC cells. GO analysis reveals that most genes correlated highly with SNAP23 were enriched in metabolic process.
CONCLUSIONS
Our data suggest that SNAP23 may serve as an oncogene promoting tumorigenicity of OC cells by decreasing apoptotic process.
背景
卵巢癌（OC）是主要的妇科恶性肿瘤，而可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）蛋白与肿瘤进展密切相关。在此，我们鉴定出SNARE复合体成员SNAP23是OC中的一种潜在致癌基因。
方法
我们测定了OC组织中SNAP23的表达，并通过生物信息学分析探讨其临床意义。随后在体外评估了SNAP23对OC细胞增殖、迁移、侵袭、细胞周期和凋亡的影响。
结果
与正常组织相比，SNAP23在OC肿瘤组织中高表达，且SNAP23表达增加与无进展生存期较差相关（风险比[HR]=1.24，95%置信区间[CI]=1.07-1.44，p=0.0042）。敲低SNAP23可增加细胞凋亡，并抑制OC细胞的增殖、迁移和侵袭。基因本体（GO）分析显示，大多数与SNAP23高度相关的基因在代谢过程中富集。
结论
我们的数据表明，SNAP23可能作为一种致癌基因，通过减少凋亡过程促进OC细胞的致瘤性。
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