Ferjani Sana, Saidani Mabrouka, Hamzaoui Zeineb, Alonso Carla Andrea, Torres Carmen, Maamar Elaa, Slim Amine Faouzi, Boutiba Ben Boubaker Ilhem
Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux antimicrobiens, 1007, Tunis, Tunisie.
Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux antimicrobiens, 1007, Tunis, Tunisie; CHU Charles Nicolle, Service de Microbiologie, 1006, Tunis, Tunisie.
Diagn Microbiol Infect Dis. 2017 Feb;87(2):188-192. doi: 10.1016/j.diagmicrobio.2016.03.008. Epub 2016 Mar 8.
The spread of extended spectrum β-lactamases (ESBL) and plasmid mediated AmpC β-lactamases (pAmpC) was evaluated in Escherichia coli strains collected from the intestinal microbiota of healthy children in Tunisia. The carriage rate of CTXE. coli was 6.6% (7 of 105 samples) and one strain/sample was further characterized (7 isolates). These isolates harbored bla (n = 4), bla (n = 2), and bla gene (n = 1), which were usually located on FIB replicon type and carried class 1 integrons. The acc(6')-Ib-cr variant was identified in one isolate that harbored bla. CTXE. coli isolates were genetically unrelated and belonged to B1 (n = 3/ST155/ST398/ST58), D (n = 2/ST117/ST493), B2 (n = 1/ST127), and A (n = 1/ST746) phylogroups. Strain virulence scores varied from 3 to 12, and frequently harbored the pathogenicity island PAI IV. The intestinal tract of healthy children constitute an important reservoir of ESBL producing E. coli. Thus, improvement of hygiene measures mainly in the school environment and rational use of antibiotics would be of great help in preventing selection and diffusion of resistant strains from intestinal microbiota.
在从突尼斯健康儿童肠道微生物群中分离出的大肠杆菌菌株中，评估了超广谱β-内酰胺酶（ESBL）和质粒介导的AmpCβ-内酰胺酶（pAmpC）的传播情况。产CTX-M大肠杆菌的携带率为6.6%（105份样本中有7份），每个样本进一步鉴定出一株菌株（共7株分离株）。这些分离株携带bla（n = 4）、bla（n = 2）和bla基因（n = 1），这些基因通常位于FIB复制子类型上，并携带1类整合子。在一株携带bla的分离株中鉴定出acc(6')-Ib-cr变体。产CTX-M大肠杆菌分离株在基因上不相关，属于B1（n = 3/ST155/ST398/ST58）、D（n = 2/ST117/ST493）、B2（n = 1/ST127）和A（n = 1/ST746）系统发育群。菌株毒力评分从3到12不等，并且经常携带致病岛PAI IV。健康儿童的肠道构成了产ESBL大肠杆菌的重要储存库。因此，主要在学校环境中改善卫生措施以及合理使用抗生素将有助于预防肠道微生物群中耐药菌株的选择和扩散。
