Pugajeva Iveta, Rusko Janis, Perkons Ingus, Lundanes Elsa, Bartkevics Vadims
Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes iela 3, Riga LV-1076, Latvia; University of Latvia, Faculty of Chemistry, Jelgavas iela 1, Riga LV-1004, Latvia.
University of Latvia, Faculty of Chemistry, Jelgavas iela 1, Riga LV-1004, Latvia.
J Pharm Biomed Anal. 2017 Jan 30;133:64-74. doi: 10.1016/j.jpba.2016.11.008. Epub 2016 Nov 10.
A multi-class method for the determination of 24 emerging pharmaceutical residues has been developed and validated. The method is based on solid-phase extraction of wastewater samples using Strata-X cartridges followed by high performance liquid chromatography coupled to hybrid quadrupole - Orbitrap high resolution mass spectrometry (HPLC-Q-Orbitrap-HRMS). A single-laboratory validation procedure showed satisfactory analytical performance. The analysis of 21 samples collected at the wastewater treatment plant in Riga revealed the occurrence of 20 compounds of different therapeutic classes. The highest concentration was found for the central nervous system stimulator caffeine - up to 12μgL, the analgesic acetaminophen up to 4.2μgL, the antibiotic ciprofloxacin in the concentration range of 250-400ngL, and the non-steroidal anti-inflammatory drug (NSAID) ibuprofen at 100-325ngL.
已开发并验证了一种用于测定24种新兴药物残留的多类别方法。该方法基于使用Strata-X柱对废水样品进行固相萃取，然后进行高效液相色谱-混合四极杆-轨道阱高分辨率质谱联用（HPLC-Q-Orbitrap-HRMS）。单实验室验证程序显示出令人满意的分析性能。对在里加污水处理厂采集的21个样品进行分析后发现，存在20种不同治疗类别的化合物。发现中枢神经系统兴奋剂咖啡因的浓度最高，可达12μg/L，镇痛药对乙酰氨基酚最高可达4.2μg/L，抗生素环丙沙星的浓度范围为250-400ng/L，非甾体抗炎药布洛芬为100-325ng/L。
