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从一只猫头鹰（穴小鸮）分离出的疫苗株新城疫病毒的全基因组序列

Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).

作者信息

Van Borm Steven, Rizotto Laís S, Ullmann Leila S, Scagion Guilherme P, Malossi Camila D, Simão Raphael M, Araújo João P, Cordeiro Izabelle M, Keid Lara B, Oliveira Trícia Maria F Sousa, Soares Rodrigo M, Martini Matheus C, Orsi Maria A, Arns Clarice W, Ferreira Helena L

机构信息

Molecular Platform Unit, CODA-CERVA Veterinary and Agrochemical Research Centre, Brussels, Belgium.

Postgraduate Program in Experimental Epidemiology of Zoonoses, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), São Paulo, Brazil.

出版信息

Genome Announc. 2016 Nov 17;4(6):e01243-16. doi: 10.1128/genomeA.01243-16.

DOI:10.1128/genomeA.01243-16
PMID:27856578
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5114370/
Abstract

A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription-PCR (RRT-PCR) from a captive owl swab. The complete genome sequence of APMV-1/Rhinoptynx clamator/Brazil/22516/2009 (APMV-1, avian paramyxovirus type 1) was obtained using Illumina sequencing. Phylogenetic analysis of the complete genome classified the isolate within NDV class II genotype II.

摘要

通过实时逆转录聚合酶链反应（RRT-PCR）从一只圈养猫头鹰的拭子中检测到新城疫病毒（NDV）后，该病毒在鸡胚中分离出来。使用Illumina测序获得了禽副粘病毒1型（APMV-1）/巴西眼镜鸮/巴西/22516/2009（APMV-1）的完整基因组序列。对完整基因组的系统发育分析将该分离株归类为NDV II类基因型II。

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