来自约旦的VIIi基因型新城疫病毒的首个基因组序列
First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan.
作者信息
Ababneh Mustafa, Ferreira Helena L, Khalifeh Mohammad, Suarez David L, Afonso Claudio L
机构信息
Department of Basic Medical Veterinary Sciences, Jordan University of Science & Technology, Irbid, Jordan.
U.S. National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, Georgia, USA.
出版信息
Microbiol Resour Announc. 2018 Dec 13;7(23). doi: 10.1128/MRA.01136-18. eCollection 2018 Dec.
Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing. Phylogenetic analysis of the concatenated coding sequences classified the virus as class II subgenotype VIIi.
通过逆转录聚合酶链反应（RT-PCR）从约旦一个后院鸡群的鸡脾脏分离的总RNA中检测到新城疫病毒（NDV）。利用MiSeq（Illumina）测序获得了NDV/鸡/约旦/J11-脾脏/2018的完整编码基因组序列。对串联编码序列的系统发育分析将该病毒归类为II类VIIi亚基因型。
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