Wikén M, Björck P, Axelsson B, Perlmann P
University of Stockholm, Department of Immunology, Sweden.
Scand J Immunol. 1989 Mar;29(3):353-61. doi: 10.1111/j.1365-3083.1989.tb01134.x.
The monoclonal antibody (MoAb) B1B6 to human leucocyte sialoglycoprotein, CD43, induces aggregation of T cells and delivers progression signals early during activation of both T and B cells in the presence of primary activators of protein kinase C. In this report we further studied the role of CD43 in human B-cell activation and differentiation. About 5-10% of resting tonsillar B cells are CD43+. In the presence of TPA or antibodies to CDw40, the proportions of CD43+ cells drastically increased. The expression was optimal on day 3 of culture, when up to 80% and 50%, respectively, were CD43+. Whereas MoAb B1B6 together with TPA induced a three- to fivefold higher proliferative response as compared to TPA alone, antibodies to CDw40 did not synergize with MoAb B1B6 in B-cell proliferation. Tonsillar populations depleted of CD43+ B cells responded with lower proliferation to TPA alone or to TPA and B1B6 or anti-CDw40 antibodies. MoAb B1B6 did not affect the production of IgM or IgG as induced by pokeweed mitogen in the presence of autologous T cells, from either peripheral blood or tonsillar B cells. Neither did it affect the IgG production from the CD43+ BSF-2 sensitive Epstein-Barr virus-transformed lymphoblastoid cell line CESS. The results show that CD43 is upregulated on B cells during activation. Furthermore, CD43+ B cells are included in the population which responds to signals delivered by TPA, anti-CD43 or anti-CDw40 antibodies, and the proliferation of this population is not merely due to an expansion of the small population of CD43+ cells present among these cells. Moreover, the epitopes recognized by MoAb B1B6 are not involved in the differentiation of and ultimate Ig-secretion from activated B cells.
针对人白细胞唾液糖蛋白CD43的单克隆抗体（MoAb）B1B6，在蛋白激酶C的初级激活剂存在的情况下，可诱导T细胞聚集，并在T细胞和B细胞激活早期传递进展信号。在本报告中，我们进一步研究了CD43在人B细胞激活和分化中的作用。约5%-10%的静息扁桃体B细胞为CD43阳性。在存在佛波酯（TPA）或抗CDw40抗体的情况下，CD43阳性细胞的比例急剧增加。培养第3天时表达最为显著，此时分别高达80%和50%的细胞为CD43阳性。与单独使用TPA相比，MoAb B1B6与TPA共同诱导的增殖反应高出三至五倍，而抗CDw40抗体在B细胞增殖中不与MoAb B1B6协同作用。去除CD43阳性B细胞的扁桃体细胞群体，对单独的TPA或TPA与B1B6或抗CDw40抗体的反应增殖较低。在存在来自外周血或扁桃体B细胞的自体T细胞的情况下，MoAb B1B6不影响商陆丝裂原诱导的IgM或IgG产生。它也不影响来自对CD43阳性B细胞刺激因子2敏感的爱泼斯坦-巴尔病毒转化的淋巴母细胞系CESS的IgG产生。结果表明，激活过程中B细胞上的CD43表达上调。此外，CD43阳性B细胞包含在对TPA、抗CD43或抗CDw40抗体传递的信号作出反应的细胞群体中，该群体的增殖不仅仅是由于这些细胞中存在的少量CD43阳性细胞的扩增。此外，MoAb B1B6识别的表位不参与活化B细胞的分化和最终的Ig分泌。
