Wikén M, Björck P, Axelsson B, Perlmann P
University of Stockholm, Department of Immunology, Sweden.
Scand J Immunol. 1989 Mar;29(3):363-70. doi: 10.1111/j.1365-3083.1989.tb01135.x.
Monoclonal antibodies (MoAb) to human leucocyte sialoglycoprotein, CD43, have been shown to deliver mitogenic signals to human T cells or to enhance T-cell proliferation induced by concanavalin A, anti-CD3 antibodies or phorbol ester. In this paper, we studied the effects of anti-CD43 MoAb B1B6 on the activation of human B cells. Anti-CD43 MoAb B1B6 was not mitogenic by itself for human B cells. However, when added together with TPA, both resting and in vivo activated tonsillar B cells, containing 5-10% and about 35% CD43+ respectively, responded with three- to fivefold higher proliferation compared to that obtained with TPA alone. A peak in the proliferative response was reached on day 3. Optimal proliferation was obtained when the antibody was present from the start of culturing. Addition of MoAb B1B6 together with a calcium ionophore, ionomycin, did not induce B-cell proliferation. Neither did mAb B1B6 sustain the growth of B cells that were already in the cell cycle, i.e. precultured with phorbol ester (PDB) and ionomycin for 3 days. The results are similar to those obtained with antibodies to CD22 and CD23 and show that early progression signals are delivered to resting B cells through CD43 in the presence of primary activators of protein kinase C.
针对人白细胞唾液糖蛋白CD43的单克隆抗体（MoAb）已被证明可向人T细胞传递促有丝分裂信号，或增强由伴刀豆球蛋白A、抗CD3抗体或佛波酯诱导的T细胞增殖。在本文中，我们研究了抗CD43单克隆抗体B1B6对人B细胞激活的影响。抗CD43单克隆抗体B1B6本身对人B细胞无促有丝分裂作用。然而，当与佛波醇酯（TPA）一起添加时，无论是静息的还是体内激活的扁桃体B细胞（分别含有5%-10%和约35%的CD43+），其增殖反应比单独使用TPA时高3至5倍。增殖反应在第3天达到峰值。从培养开始就存在抗体时可获得最佳增殖效果。将单克隆抗体B1B6与钙离子载体离子霉素一起添加不会诱导B细胞增殖。单克隆抗体B1B6也不能维持已经处于细胞周期中的B细胞的生长，即先用佛波酯（PDB）和离子霉素预培养3天的B细胞。这些结果与用抗CD22和抗CD23抗体获得的结果相似，表明在蛋白激酶C的初级激活剂存在的情况下，早期进展信号通过CD43传递给静息B细胞。
