Sakata Masanobu, Kawasaki Tamami, Shibue Toshimichi, Namiki Hideo
Department of Integrative Bioscience and Biomedical Engineering, Waseda University, 2-2 Wakamatsucho, Shinjuku-ku, Tokyo 162-8480, Japan.
Advanced Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Biophysics (Nagoya-shi). 2010 Dec 7;6:53-57. doi: 10.2142/biophysics.6.53. eCollection 2010.
Ferromagnetic resonance (FMR) with an electron spin resonance (ESR) apparatus was investigated for super-paramagnetic particles within resting eggs. High-field (HF) resonance lines near g=2 resulted from single superparamagnetic particles, were detected from ESR spectra of resting eggs. The size of isolated superparamagnetic particles within resting eggs was calculated to be approximately 13 nm in diameter by analysis of the temperature dependence of the HF line width. Small-angle X-ray scattering (SAXS) analysis of resting eggs also showed that average size of superparamagnetic particles in diameter, equivalent to magnetite, was approximately 13 nm. The combination of FMR and SAXS measurement is very effective in estimating the size of superparamagnetic particles in biological organisms, with difficulties of preparing for samples for measurement by electron microscopy. However, , with that were raised, did not show FMR spectra, showing no magnetic particles within resting eggs. Therefore, it suggested that superparamagnetic particles within resting eggs, were mineralized in as the result of biomineralization of Fe originated from .
利用电子自旋共振（ESR）装置对休眠卵内的超顺磁性颗粒进行了铁磁共振（FMR）研究。在g = 2附近的高场（HF）共振线由单个超顺磁性颗粒产生，从休眠卵的ESR光谱中检测到。通过分析HF线宽的温度依赖性，计算出休眠卵内孤立超顺磁性颗粒的直径约为13 nm。对休眠卵的小角X射线散射（SAXS）分析也表明，相当于磁铁矿的超顺磁性颗粒的平均直径约为13 nm。FMR和SAXS测量相结合在估计生物体内超顺磁性颗粒的大小时非常有效，而制备用于电子显微镜测量的样品存在困难。然而，所培养的[此处原文缺失部分内容]没有显示FMR光谱，表明休眠卵内没有磁性颗粒。因此，这表明休眠卵内超顺磁性颗粒是由于源自[此处原文缺失部分内容]的铁的生物矿化作用而在[此处原文缺失部分内容]中矿化形成的。
Zotero 插件
