Institut Charles Gerhardt de Montpellier, MACS, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France and CEA, Direction de la Recherche Fondamentale-BIAM, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France.
CEA, Direction de la Recherche Fondamentale-IBITECS, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France.
Nanoscale. 2017 Feb 2;9(5):1840-1851. doi: 10.1039/c6nr04765c.
Magnetic mesoporous silica nanoparticles (M-MSNs) represent promising targeting tools for theranostics. Engineering the interaction of nanoparticles (NPs) with biological systems requires an understanding of protein corona formation around the nanoparticles as this drives the biological fate of nanocarriers. We investigated the behavior of proteins in contact with M-MSNs by high-throughput comparative proteomics, using human and bovine sera as biological fluids, in order to assess the adsorption dynamics of proteins in these media. Using system biology tools, and especially protein-protein interaction databases, we demonstrated how the protein network builds up within the corona over the course of the experiment. Based on these results, we introduce and discuss the role of the "corona interactome" as an important factor influencing protein corona evolution. The concept of the "corona interactome" is an original methodology which could be generalized to all NP candidates. Based on this, pre-coating nanocarriers with specific proteins presenting minimal interactions with opsonins might provide them with properties such as stealth.
磁性介孔硅纳米颗粒（M-MSNs）是一种很有前途的治疗诊断靶向工具。为了设计纳米颗粒（NPs）与生物系统的相互作用，需要了解纳米颗粒周围蛋白质冠的形成情况，因为这决定了纳米载体的生物学命运。我们使用人血清和牛血清作为生物流体，通过高通量比较蛋白质组学研究了与 M-MSNs 接触的蛋白质的行为，以评估这些介质中蛋白质的吸附动力学。我们使用系统生物学工具，特别是蛋白质-蛋白质相互作用数据库，证明了在实验过程中，蛋白质网络如何在冠层内建立起来。基于这些结果，我们介绍并讨论了“冠冕相互作用组”作为影响蛋白质冠冕演化的重要因素的作用。“冠冕相互作用组”的概念是一种原始的方法，可以推广到所有的 NP 候选物。基于这一点，用与调理素相互作用最小的特定蛋白质预先涂覆纳米载体，可能会赋予它们隐身等特性。
