Institut Charles Gerhardt de Montpellier, MACS, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France.
Nanoscale. 2017 May 11;9(18):5769-5772. doi: 10.1039/c7nr01654a.
In order to direct nanocarriers to their targets efficiently, we have to understand the interactions occurring at the nano-bio interface between nanocarriers and human proteins, which forms the layer called the corona. However, experiments aiming to identify and quantify the proteins in the corona, especially critical steps in the separation of nanoparticles from biological media may affect the corona composition. Here, we used nano-LC MS/MS to compare the protein corona contents obtained after using two different separation methods. We showed that applying centrifugation versus magnetization to isolate nanoparticles surrounded by a corona resulted in protein loss and a reshuffling of their respective abundances.
为了有效地将纳米载体导向其靶标，我们必须了解纳米载体与人体蛋白之间在纳米-生物界面发生的相互作用，这些相互作用形成了所谓的“冠层”。然而，旨在鉴定和量化冠层中蛋白质的实验，特别是在将纳米颗粒从生物介质中分离出来的关键步骤，可能会影响冠层的组成。在这里，我们使用纳升 LC-MS/MS 比较了使用两种不同分离方法获得的蛋白质冠层含量。我们表明，离心与磁化相比，用于分离被冠层包围的纳米颗粒会导致蛋白质损失和各自丰度的重新分配。
