[不同剂量辐射对人涎腺腺样囊性癌细胞的影响]
[Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells].
作者信息
Huang Yu-Meng, Wang Ying, Li Yong, Liu Ping, Liu Xian-Feng, Zhao Hong-Wei
机构信息
Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University. Chongqing 401147,China. E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2016 Aug;25(4):403-408.
PURPOSE
To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells (SACC-83, SACC-LM).
METHODS
Different doses of radiation (0, 2, 4, 6, 8, 10 Gy) were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry(FCM) and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis.
RESULTS
The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells (P<0.05). In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability.
CONCLUSIONS
Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.
目的
探讨不同剂量辐射对人涎腺腺样囊性癌细胞（SACC - 83、SACC - LM）的影响。
方法
对SACC - 83、SACC - LM细胞施加不同剂量的辐射（0、2、4、6、8、10 Gy），并将细胞继续培养48小时。采用CCK - 8检测、流式细胞术（FCM）和细胞划痕实验观察细胞增殖、凋亡及细胞迁移情况。使用SPSS19.0软件包进行数据分析。
结果
辐射对SACC - LM细胞存活率、细胞凋亡、异倍体及细胞迁移能力的影响显著大于对SACC - 83细胞的影响（P<0.05）。在SACC - 83细胞中，与其他剂量辐射相比，6 Gy辐射对细胞存活率、细胞凋亡、异倍体及细胞迁移能力最为敏感。
结论
SACC - LM的辐射敏感性大于SACC - 83细胞。6 Gy辐射时，SACC - 83细胞的生物学变化比其他剂量辐射更为显著。
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