[CCL5/CCR5在涎腺腺样囊性癌细胞神经侵袭中的作用]
[Role of CCL5/CCR5 in the perineural invasion of salivary adenoid cystic carcinoma cells].
作者信息
Tianyi Li, Zhiyuan Shen
机构信息
Dept. of Pediatric Dentistry, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Oct 1;35(5):479-483. doi: 10.7518/hxkq.2017.05.006.
OBJECTIVE
This study aimed the role of the CCL5/CCR5 axis in the perineural invasion of salivary adenoid cystic carcinoma (SACC) cells.
METHODS
Immunohistochemical analysis and flow cytometric analysis were conducted to detect the expression of the chemokine receptor CCR5 in SACC cells. Enzyme linked immunosorbent assay (ELISA) was performed to determine the expression of CCL5 in the supernate of human nerve cells. The flow cytometric analysis was applied to observe the changes in F-actin in SACC-LM cells, which were pretreated with CCL5. To assess the effects of the CCL5/CCR5 axis on the migration and invasion of SACC-LM cells, we performed a scratch test and invasion assay under CCL5 stimulation.
RESULTS
CCR5 was highly expressed in SACC cells. The concentration of CCL5 in the supernatant of human nerve cells was (359.2±15.8), (696.4±22.6) pg·mL⁻¹. The CCL5/CCR5 axis promoted the migration and invasion of SACC-LM cells.
CONCLUSIONS
The CCL5/CCR5 axis may be involved in the perineural invasion of SACC cells.
目的
本研究旨在探讨CCL5/CCR5轴在涎腺腺样囊性癌（SACC）细胞神经侵犯中的作用。
方法
采用免疫组化分析和流式细胞术检测SACC细胞中趋化因子受体CCR5的表达。采用酶联免疫吸附测定（ELISA）法检测人神经细胞上清液中CCL5的表达。应用流式细胞术观察经CCL5预处理的SACC-LM细胞中F-肌动蛋白的变化。为评估CCL5/CCR5轴对SACC-LM细胞迁移和侵袭的影响，我们在CCL5刺激下进行了划痕试验和侵袭试验。
结果
CCR5在SACC细胞中高表达。人神经细胞上清液中CCL5的浓度为（359.2±15.8）、（696.4±22.6）pg·mL⁻¹。CCL5/CCR5轴促进了SACC-LM细胞的迁移和侵袭。
结论
CCL5/CCR5轴可能参与了SACC细胞的神经侵犯。
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