Clark C Graham, Stensvold C Rune
Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Department of Microbiology and Infection, Statens Serum Institut, Copenhagen, Denmark.
Curr Protoc Microbiol. 2016 Nov 18;43:20A.1.1-20A.1.8. doi: 10.1002/cpmc.18.
Blastocystis is an intestinal parasite that is very easily isolated in culture from fresh stool samples. In fact, the parasite grows so readily in culture that short-term in vitro culture is sometimes used as a diagnostic tool in the absence of DNA-based methods. While axenizing Blastocystis cultures remains a significant challenge, the parasite can be propagated for several months in the presence of metabolically active bacteria (xenic culture). Hence, culture can be used for maintaining live Blastocystis strain libraries. This enables the production of a stable resource of reference material, which for instance can be used for DNA-based assays and research. Blastocystis isolates can also be cryopreserved with a view to reestablishing them in culture. Here, we provide protocols for xenic in vitro culture and cryopreservation of Blastocystis. © 2016 by John Wiley & Sons, Inc.
芽囊原虫是一种肠道寄生虫，很容易从新鲜粪便样本中通过培养分离出来。事实上，这种寄生虫在培养中生长非常容易，以至于在没有基于DNA的方法时，短期体外培养有时被用作一种诊断工具。虽然使芽囊原虫培养物无菌化仍然是一项重大挑战，但该寄生虫在有代谢活性细菌存在的情况下（共栖培养）可以繁殖数月。因此，培养可用于维持活的芽囊原虫菌株库。这使得能够产生稳定的参考材料资源，例如可用于基于DNA的检测和研究。芽囊原虫分离株也可以冷冻保存，以便在培养中重新建立。在此，我们提供芽囊原虫共栖体外培养和冷冻保存的方案。© 2016约翰威立国际出版公司
