Department of Microbiology, SydPath, St. Vincent's Hospital, Sydney, Australia.
Am J Trop Med Hyg. 2011 Feb;84(2):308-12. doi: 10.4269/ajtmh.2011.10-0447.
We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp.
我们使用五种诊断技术检测了来自澳大利亚悉尼的 513 名患者的粪便样本，以检测 Blastocystis：使用改良的铁苏木精染色对永久性染色涂片进行显微镜检查、两种 Xenic 培养系统（改良的 Boeck 和 Drbohlav 培养基和胰蛋白胨、酵母提取物、葡萄糖、蛋氨酸-9 培养基），以及两种已发表的针对小亚基核糖体 DNA 的常规聚合酶链反应方法。在一种或多种诊断技术中，有 98 个（19%）样本呈 Blastocystis 阳性。PCR2 方法的检测敏感性最高，为 94%，而永久性染色显微镜检查的敏感性最低（48%）。亚型 3 是最主要的亚型（存在于 43%被分配到该组的样本中）。这项研究强调了在作为检测 Blastocystis sp.的唯一诊断方法时，显微镜检查的敏感性较低。
