Elder Steven, Pinheiro Amanda, Young Christian, Smith Preston, Wright Emily
Department of Agricultural and Biological Engineering, Mississippi State University, P.O. Box 9632, Starkville 39762, Mississippi.
Department of Biomedical Engineering, The University of Akron, Akron 44325, Ohio.
J Orthop Res. 2017 Sep;35(9):1949-1957. doi: 10.1002/jor.23483. Epub 2017 Mar 24.
We speculate that an acellular osteochondral xenograft may be a good alternative to allografts for repair of focal articular cartilage lesions. In order to make a xenograft resistant to enzymatic degradation and to prevent a chronic immune response it may be beneficial to stabilize it through crosslinking. The concept is analogous to treatment of porcine bioprosthetic heart valves with glutaraldehyde. The purpose of this study was to evaluate genipin, a natural crosslinking agent with low cytotoxicity, for stabilization of decellularized cartilage. Porcine articular cartilage discs were decellularized in SDS and nucleases and then crosslinked in genipin. The utility of genipin was determined from its effects on degree of crosslinking, mechanical properties, dimensional stability, enzymatic resistance, and in vitro biocompatibility. Degree of crosslinking, compressive moduli, and collagenase resistance varied over a wide range depending on genipin concentration. The equilibrium compressive modulus could be increased from approximately 50% to more than 120% that of native cartilage, and the time to complete degradation by collagenase could be extended from less than 12 h to more than 15 days. Radial shrinkage of approximately 4% was observed at a genipin concentration of 0.1% wt/vol, and cartilage coefficient of friction against glass increased in a concentration-dependent manner. Autologous chondrocytes displayed little difference in viability or their ability to attach and spread over the surface of genipin-fixed cartilage compared to non-crosslinked cartilage during 6 weeks of culture. These results indicate that genipin may be efficacious for stabilization of a decellularized porcine osteochondral xenograft. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1949-1957, 2017.
我们推测，对于修复局灶性关节软骨损伤，脱细胞骨软骨异种移植物可能是同种异体移植物的一个良好替代选择。为了使异种移植物具有抗酶降解能力并防止慢性免疫反应，通过交联对其进行稳定处理可能是有益的。这一概念类似于用戊二醛处理猪生物人工心脏瓣膜。本研究的目的是评估京尼平（一种具有低细胞毒性的天然交联剂）对脱细胞软骨的稳定作用。将猪关节软骨盘在SDS和核酸酶中进行脱细胞处理，然后用京尼平进行交联。根据京尼平对交联程度、力学性能、尺寸稳定性、酶抗性和体外生物相容性的影响来确定其效用。交联程度、压缩模量和抗胶原酶能力随京尼平浓度的变化范围很广。平衡压缩模量可从天然软骨的约50%增加到超过120%，胶原酶完全降解所需的时间可从少于12小时延长到超过15天。在京尼平浓度为0.1%（重量/体积）时观察到约4%的径向收缩，软骨与玻璃之间的摩擦系数随浓度增加而增加。在6周的培养过程中，与未交联的软骨相比，自体软骨细胞在京尼平固定的软骨表面的活力、附着和铺展能力几乎没有差异。这些结果表明，京尼平可能对稳定脱细胞猪骨软骨异种移植物有效。©2017骨科研究协会。由威利期刊公司出版。《矫形外科学研究》35:1949 - 1957, 2017。
