Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.
Department of Chemical, Materials and Industrial Production Engineering University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.
J Tissue Eng Regen Med. 2018 Jan;12(1):e71-e81. doi: 10.1002/term.2350. Epub 2017 Mar 27.
Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, as they represent a biomimetic environment in which cells can recapitulate certain behaviours that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue. However, these processes are complex, time-consuming and expensive. In this work, we propose a cost-effective, easy-to-produce decellularized dermal matrix, derived from animal skin. The chemical/physical processes to obtain the matrices proved to not alter matrix structure and did not induce cytotoxicity issues. To test the validity of the decellularized matrices as a model to study the behaviour of tumour cells in vitro, we performed microstructural and mechanical investigations as well as cell proliferation assays. In particular, three different tumour cell lines were used, which proliferated and invaded the matrix with no additional treatments. Decellularized skin scaffold, presented in this work, could be a strong competitor for conventional 3D systems like synthetic porous scaffolds or hydrogels. Copyright © 2016 John Wiley & Sons, Ltd.
去细胞基质在研究细胞和组织生物学方面的应用越来越受到关注，因为它们代表了一种仿生环境，细胞可以在其中再现与体内观察到的某些行为相似的行为。基本上，去细胞基质的生物化学、微观结构和力学是将这些培养系统与传统二维模型区分开来的最有价值的特性。迄今为止，已经提出了几种去细胞化组织的方法，其共同目的是保留原始组织的组织化学/物理特性。然而，这些过程复杂、耗时且昂贵。在这项工作中，我们提出了一种经济高效、易于生产的源自动物皮肤的去细胞真皮基质。获得基质的化学/物理过程证明不会改变基质结构，也不会引起细胞毒性问题。为了测试去细胞基质作为体外研究肿瘤细胞行为的模型的有效性，我们进行了微观结构和机械研究以及细胞增殖测定。特别是，使用了三种不同的肿瘤细胞系，它们在没有额外处理的情况下增殖并侵入基质。本文介绍的去细胞皮肤支架可以成为合成多孔支架或水凝胶等传统 3D 系统的有力竞争者。版权所有©2016 约翰威立父子公司
