Kang Yifei, Xue Yansong, Du Min, Zhu Mei-Jun
School of Food Science, Washington State University, Pullman, WA, 99164, USA.
Department of Animal Science, Washington State University, Pullman, WA, 99164, USA.
J Nutr Biochem. 2017 Feb;40:70-76. doi: 10.1016/j.jnutbio.2016.10.009. Epub 2016 Oct 27.
Goji berry (Lycium barbarum) exerts immune modulation and suppresses inflammation in vitro and in vivo. We hypothesized that Goji berry had beneficial effects on dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice through suppressing inflammation. Six-week-old male C57BL/6 mice were supplemented with a standard AIN-93G diet with or without 1% (w/w) Goji berry for 4 weeks. Then, colitis was induced by supplementing 3% DSS in drinking water for 7 days, followed by 7 days of remission period to mimic ulcerative colitis symptoms. Goji berry supplementation ameliorated DSS-induced body weight loss, diminished diarrhea and gross bleeding, and resulted in a significantly decreased disease activity index, as well as DSS-associated colon shortening. Moreover, 30% mortality rate caused by DSS-induced colitis was avoided because of Goji berry supplementation. Histologically, Goji berry ameliorated colonic edema, mucosal damage and neutrophil infiltration into colonic intestinal tissue in response to DSS challenge, which was associated with decreased expression of chemokine (C-X-C motif) ligand 1 and monocyte chemoattractant protein-1, as well as inflammatory mediators interleukin-6 and cyclooxygenase-2. In conclusion, Goji supplementation confers protective effects against DSS-induced colitis, which is associated with decreased neutrophil infiltration and suppressed inflammation. Thus, dietary Goji is likely beneficial to inflammatory bowel disease patients as a complementary therapeutic strategy.
枸杞（宁夏枸杞）在体外和体内均具有免疫调节作用并能抑制炎症。我们推测枸杞通过抑制炎症对葡聚糖硫酸钠（DSS）诱导的C57BL/6小鼠结肠炎具有有益作用。六周龄雄性C57BL/6小鼠补充含或不含1%（w/w）枸杞的标准AIN-93G饮食，持续4周。然后，通过在饮用水中补充3% DSS诱导结肠炎7天，随后经过7天缓解期以模拟溃疡性结肠炎症状。补充枸杞可改善DSS诱导的体重减轻，减少腹泻和明显出血，并导致疾病活动指数显著降低，以及与DSS相关的结肠缩短。此外，由于补充枸杞，避免了DSS诱导的结肠炎导致的30%死亡率。组织学上，枸杞改善了结肠水肿、黏膜损伤以及DSS刺激后中性粒细胞向结肠肠组织的浸润，这与趋化因子（C-X-C基序）配体1和单核细胞趋化蛋白-1以及炎症介质白细胞介素-6和环氧化酶-2的表达降低有关。总之，补充枸杞对DSS诱导的结肠炎具有保护作用，这与中性粒细胞浸润减少和炎症抑制有关。因此，食用枸杞作为一种辅助治疗策略可能对炎症性肠病患者有益。
