School of Food Science, Washington State University, Pullman, WA 99164, USA.
Department of Animal Science, Washington State University, Pullman, WA 99164, USA.
J Nutr Biochem. 2018 Jan;51:40-46. doi: 10.1016/j.jnutbio.2017.08.017. Epub 2017 Sep 14.
Persistent intestinal inflammation severely impairs intestinal integrity resulting in inflammatory bowel disease. Red raspberries (RB) are a rich source of bioactive compounds; their beneficial effect on the colitis protection was evaluated in the current study using a dextran sulfate sodium (DSS)-induced acute colitis mouse model. Six-week-old mice were fed a standard rodent research diet supplemented with RB (0 or 5% w/w, n=20 each group) for 6 weeks. At the 4th week of dietary treatment, approximately half of mice in each dietary group (n=12 each group) were subjected to 2.5% DSS induction for 6 days, followed by 6 days of recovery, to induce colitis. RB supplementation decreased body weight loss (P≤.01), disease activity index (P≤.01), and colon shortening (P≤.05) in DSS-treated mice. In addition, RB supplementation protected the colonic structure (P≤.01), associated with suppressed NF-κB signaling and reduced expression of inflammatory interleukin (IL)-1β, IL-6, IL-17, cyclooxegenase-2, and tumor necrosis factor-α in DSS-treated mice. RB supplementation reduced neutrophil infiltration, monocyte chemoattractant protein-1 mRNA expression, and xanthine oxidase content, but enhanced catalase content in DSS-treated mice. Consistently, RB supplementation reduced pore forming tight junction protein claudin-2, increased barrier strengthening claudin-3, zonula occluden-1 protein content and mucin (MUC)-2 mRNA level, and activated AMP-activated protein kinase (AMPK) in DSS-treated mice. In conclusion, dietary RB protected against inflammation and colitis symptoms induced by DSS, providing a promising dietary approach for the management of colitis.
持续的肠道炎症严重损害肠道完整性，导致炎症性肠病。红树莓（RB）是生物活性化合物的丰富来源；本研究采用葡聚糖硫酸钠（DSS）诱导的急性结肠炎小鼠模型，评估了其对结肠炎保护的有益作用。6 周龄小鼠用标准啮齿动物研究饮食喂养，并用 RB（0 或 5%w/w，每组 20 只）补充 6 周。在饮食治疗的第 4 周，每组约一半的小鼠（每组 12 只）接受 2.5%DSS 诱导 6 天，随后进行 6 天的恢复期，以诱导结肠炎。RB 补充剂可减少 DSS 处理小鼠的体重减轻（P≤.01）、疾病活动指数（P≤.01）和结肠缩短（P≤.05）。此外，RB 补充剂保护结肠结构（P≤.01），与 NF-κB 信号抑制和炎性白细胞介素（IL）-1β、IL-6、IL-17、环氧化酶-2 和肿瘤坏死因子-α表达降低相关在 DSS 处理的小鼠中。RB 补充剂减少了中性粒细胞浸润、单核细胞趋化蛋白-1 mRNA 表达和黄嘌呤氧化酶含量，但增加了 DSS 处理小鼠中的过氧化氢酶含量。一致地，RB 补充剂降低了孔形成紧密连接蛋白 Claudin-2，增加了屏障强化 Claudin-3、Zonula Occluden-1 蛋白含量和粘蛋白（MUC）-2 mRNA 水平，并激活了 DSS 处理小鼠中的 AMP 激活蛋白激酶（AMPK）。总之，饮食 RB 可预防 DSS 诱导的炎症和结肠炎症状，为结肠炎的管理提供了一种有前景的饮食方法。
