Czyzewicz Nathan, Stes Elisabeth, De Smet Ive
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Department of Plant Systems Biology, Flemish Institute of Biotechnology (VIB), 9052, Ghent, Belgium.
Methods Mol Biol. 2017;1497:19-28. doi: 10.1007/978-1-4939-6469-7_3.
The first signaling peptide discovered and purified was insulin in 1921. However, it was not until 1991 that the first peptide signal, systemin, was discovered in plants. Since the discovery of systemin, peptides have emerged as a potent and diverse class of signaling molecules in plant systems. Peptides consist of small amino acid sequences, which often act as ligands of receptor kinases. However, not all peptides are created equal, and signaling peptides are grouped into several subgroups dependent on the type of post-translational processing they undergo. Here, we focus on the application of synthetic, post-translationally modified peptides (PTMPs) to plant systems, describing several methods appropriate for the use of peptides in Arabidopsis thaliana and crop models.
1921年发现并纯化的首个信号肽是胰岛素。然而，直到1991年才在植物中发现首个肽信号——系统素。自系统素被发现以来，肽已成为植物系统中一类强大且多样的信号分子。肽由小的氨基酸序列组成，这些序列通常作为受体激酶的配体。然而，并非所有肽都是相同的，信号肽根据它们所经历的翻译后加工类型被分为几个亚组。在此，我们聚焦于合成的、翻译后修饰肽（PTMPs）在植物系统中的应用，描述几种适用于在拟南芥和作物模型中使用肽的方法。
