Lillehoj H S, Kang S Y, Keller L, Sevoian M
Protozoan Diseases Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Exp Parasitol. 1989 Jul;69(1):54-64. doi: 10.1016/0014-4894(89)90171-9.
The role of avian lymphokines as nonspecific immunomodulators of host immunity against the intracellular parasite Eimeria was investigated. Prophylactic treatment of normal chickens with crude cell-free supernatants obtained from JMV-1 culture, concanavalin A (Con A)-stimulated normal spleen cells, or sporozoite-stimulated immune T cells prior to inoculation with E. tenella or E. acervulina conferred significant protection. These crude cell-free culture supernatants also inhibited intracellular development of eimerian parasites in vitro. Avian macrophages pretreated with these supernatant preparations showed inhibitory activity against Eimeria. This inhibitory activity could not be ascribed to anti-Eimeria antibody, complement, or cell-free Marek's disease virus and was therefore considered to be due to immunomodulating lymphokines present in the culture supernatants. These results suggest that JMV-1-transformed T lymphoblastoid cells, immune T lymphocytes, and Con A-stimulated normal spleen cells secrete lymphokines that can enhance host immunity in a nonspecific manner and implicate cell-mediated immunity as a major mechanism of the protective host immune response against eimerian infections.
研究了禽类淋巴因子作为宿主针对细胞内寄生虫艾美耳球虫的非特异性免疫调节剂的作用。在用柔嫩艾美耳球虫或堆型艾美耳球虫接种之前，用从JMV-1培养物、刀豆球蛋白A（Con A）刺激的正常脾细胞或子孢子刺激的免疫T细胞获得的无细胞粗上清液对正常鸡进行预防性处理，可提供显著的保护作用。这些无细胞粗培养上清液在体外也抑制艾美耳球虫寄生虫的细胞内发育。用这些上清液制剂预处理的禽类巨噬细胞显示出对艾美耳球虫的抑制活性。这种抑制活性不能归因于抗艾美耳球虫抗体、补体或无细胞马立克氏病病毒，因此被认为是由于培养上清液中存在的免疫调节淋巴因子。这些结果表明，JMV-1转化的T淋巴母细胞、免疫T淋巴细胞和Con A刺激的正常脾细胞分泌的淋巴因子可以非特异性地增强宿主免疫力，并表明细胞介导的免疫是宿主针对艾美耳球虫感染的保护性免疫反应的主要机制。
