Lillehoj H S, Jenkins M C, Bacon L D, Fetterer R H, Briles W E
Livestock and Poultry Science Institute, USDA-ARS, Beltsville, Maryland 20705.
Exp Parasitol. 1988 Dec;67(2):148-58. doi: 10.1016/0014-4894(88)90062-8.
The roles of major histocompatibility complex (MHC) and non-MHC-linked genes in the genetic control of disease susceptibility and the development of protective immunity to Eimeria acervulina infection were investigated in six 15I5-B congenic and four different strains of chickens characterized for the MHC. When oocyst production was assessed, wide variations were noted following initial and challenge infections among the strains of chickens tested. In general, 15.N-21, 15.P-13, B21, B19, SC, and FP chickens were protected following challenge infection whereas 15I5, 15.P-19, 15.7-2, and 15.6-2 chickens were not. Strains of chickens sharing a same B haplotype on different genetic backgrounds did not show comparable levels of protection. These results lead to the view that non-MHC-linked genes have a profound influence on the outcome of the host response to E. acervulina infection. Chickens infected twice at 1-month intervals by an oral inoculation with E. acervulina developed both coccidial-specific antibody and T-cell responses. E. acervulina infected chickens showed T-cell-mediated immune responses to the intact sporozoites as well as to recombinant proteins, p130 of sporozoites and p150 of merozoites. Both p130 and p150 antigens have been identified and characterized previously. Sera obtained from all infected chickens recognized the p150 merozoite protein, but not the p130 sporozoite protein in immunoblots. In general, the cellular response, but not the antibody response to the p150 recombinant surface merozoite antigen correlated with the degree of protection following the challenge infection. These results suggest that the strains of chickens having improved protection against challenge infection demonstrate higher T-cell responses to the recombinant surface merozoite protein, p150.
研究了主要组织相容性复合体（MHC）和非MHC连锁基因在疾病易感性的遗传控制以及对堆型艾美耳球虫感染的保护性免疫发育中的作用，所用材料为六个15I5 - B同源基因系和四个具有不同MHC特征的鸡品系。在评估卵囊产量时，发现受试鸡品系在初次感染和再次感染后存在广泛差异。一般来说，15.N - 21、15.P - 13、B21、B19、SC和FP鸡在再次感染后受到保护，而15I5、15.P - 19、15.7 - 2和15.6 - 2鸡则未受到保护。在不同遗传背景下共享相同B单倍型的鸡品系并未表现出相当的保护水平。这些结果表明，非MHC连锁基因对宿主对堆型艾美耳球虫感染的反应结果有深远影响。通过口服接种堆型艾美耳球虫，每隔1个月感染两次的鸡产生了球虫特异性抗体和T细胞反应。感染堆型艾美耳球虫的鸡对完整子孢子以及重组蛋白（子孢子的p130和裂殖子的p150）表现出T细胞介导的免疫反应。p130和p150抗原先前已被鉴定和表征。在免疫印迹中，从所有感染鸡获得的血清识别裂殖子蛋白p150，但不识别子孢子蛋白p130。一般来说，对p150重组裂殖子表面抗原的细胞反应而非抗体反应与再次感染后的保护程度相关。这些结果表明，对再次感染具有更好保护作用的鸡品系对重组裂殖子表面蛋白p150表现出更高的T细胞反应。
