Piras Enrica, Madeddu Maria A, Palmieri Giuseppina, Angius Fabrizio, Contini Pierpaolo, Pompei Raffaello, Ingianni Angela
Dipartimento di Scienze Biomediche, Università di Cagliari, via Porcell 4, 0124, Cagliari, Italy.
Policlinico Universitario, Cittadella di Monserrato, Monserrato, Cagliari, Italy.
Adv Exp Med Biol. 2017;973:41-51. doi: 10.1007/5584_2016_73.
The prevalence of Human Herpesvirus 8 (HHV8) DNA and antiviral antibodies in Diabetes type 2 (DM2) and control subjects was studied, in order to confirm a possible link between DM2 and HHV8 infection. The HHV8-DNA from diabetic patients was typed for detecting possible genomic differences with known HHV8 reference viruses.DM2 patients and healthy controls were examined for the presence of HHV8 DNA into the peripheral blood lymphocytes. Both anti-lytic and latent phase antibodies were detected in HHV8 positive and negative diabetic patients, as well in a number of controls. The HHV8 ORF K1 and ORF 26 genes from DM2 patients were typed and matched to reference strains.A significant prevalence of HHV8 DNA in DM2 subjects versus healthy controls was detected (about 58 % against 27 %). Anti-lytic phase, but not anti-latent phase antibodies, were significantly increased in DM2 patients versus controls. In addition, about 30 % of HHV8 strains isolated from DM2 lymphocytes showed consistent differences in the ORF 26 gene sequence, so that a new HHV8 subtype was proposed. These findings give additional support to the hypothesis that HHV8 could be considered an additional risk factor for DM2 onset.
为了证实2型糖尿病（DM2）与人类疱疹病毒8型（HHV8）感染之间可能存在的联系，研究了DM2患者和对照受试者中HHV8 DNA及抗病毒抗体的流行情况。对糖尿病患者的HHV8-DNA进行分型，以检测其与已知HHV8参考病毒可能存在的基因组差异。检测DM2患者和健康对照外周血淋巴细胞中HHV8 DNA的存在情况。在HHV8阳性和阴性的糖尿病患者以及一些对照中均检测到抗裂解期和潜伏期抗体。对DM2患者的HHV8 ORF K1和ORF 26基因进行分型并与参考菌株匹配。结果发现，DM2受试者中HHV8 DNA的流行率显著高于健康对照（约58%对27%）。与对照相比，DM2患者的抗裂解期抗体显著增加，但抗潜伏期抗体无显著增加。此外，从DM2淋巴细胞中分离出的约30%的HHV8菌株在ORF 26基因序列上表现出一致的差异，因此提出了一种新的HHV8亚型。这些发现进一步支持了HHV8可能是DM2发病的一个额外危险因素的假说。
