Patrick C C, Pelzel S E, Miller E E, Haanes-Fritz E, Radolf J D, Gulig P A, McCracken G H, Hansen E J
Department of Microbiology, University of Texas, Southwestern Medical Center, Dallas 75235.
Infect Immun. 1989 Jul;57(7):1971-8. doi: 10.1128/iai.57.7.1971-1978.1989.
Isolates of Haemophilus influenzae type b (Hib) can be divided into three antigenic groups based on their reactivities with a set of two monoclonal antibodies (MAbs) directed against epitopes in the oligosaccharide region of Hib lipooligosaccharide (LOS) (P. A. Gulig, C. C. Patrick, L. Hermanstorfer, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 55:513-520, 1987). Approximately 24% of Hib strains react with both of these LOS-specific MAbs. Immunoprecipitation experiments involving several of these strains indicated that the epitopes recognized by these MAbs resided in two different LOS molecules, both of which were synthesized by these particular Hib strains. In addition, Western blot (immunoblot) analysis of proteinase K-treated cell extracts of these strains that had been subjected to sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis revealed two different LOS staining patterns when they were probed independently with the two MAbs. Colony blot radioimmunoassay of hundreds of colonies of one of these Hib strains showed that each colony bound both MAbs. Immune electron microscopy confirmed that individual cells of this same Hib strain expressed both types of LOS molecule at the same time. An antibody accessibility radioimmunoassay was used to show that different Hib strains of this type varied in the relative amounts of each of the two MAbs that they could bind to their cell surfaces. These findings indicate that some Hib strains can synthesize two antigenically distinct LOS molecules simultaneously.
b型流感嗜血杆菌（Hib）分离株可根据其与一组两种针对Hib脂寡糖（LOS）寡糖区域表位的单克隆抗体（MAb）的反应性分为三个抗原组（P.A.Gulig、C.C.Patrick、L.Hermanstorfer、G.H.McCracken,Jr.和E.J.Hansen，《感染与免疫》55:513 - 520,1987）。约24%的Hib菌株与这两种LOS特异性MAb都发生反应。涉及其中几种菌株的免疫沉淀实验表明，这些MAb识别的表位存在于两种不同的LOS分子中，这两种分子均由这些特定的Hib菌株合成。此外，对经蛋白酶K处理的这些菌株的细胞提取物进行十二烷基硫酸钠 - 聚丙烯酰胺梯度凝胶电泳后的蛋白质印迹（免疫印迹）分析显示，当用这两种MAb分别探测时，呈现出两种不同的LOS染色模式。对其中一种Hib菌株的数百个菌落进行菌落印迹放射免疫测定表明，每个菌落都能结合这两种MAb。免疫电子显微镜证实，同一Hib菌株的单个细胞同时表达两种类型的LOS分子。一种抗体可及性放射免疫测定用于表明，这种类型的不同Hib菌株在其细胞表面能够结合的两种MAb的相对量上存在差异。这些发现表明，一些Hib菌株能够同时合成两种抗原性不同的LOS分子。
