Khan Imran, Ray Dutta Jayati, Ganesan Ramakrishnan
Department of Biological Sciences, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad 500078, Telangana, India.
Department of Biological Sciences, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad 500078, Telangana, India.
Int J Biol Macromol. 2017 Feb;95:126-131. doi: 10.1016/j.ijbiomac.2016.11.040. Epub 2016 Nov 16.
Polymer degradation through lipase appears to be an enthralling alternative to bulk chemical routes. Poly (ε-caprolactone) (PCL) is an artificial polyester that can be degraded by microbes and enzymes like lipases and esterases. The environmental degradation of PCL is dependent on the activity of bacteria that characterization techniques such as thermogravimetric analysis, differential thermal are widely present in the ecosystem. In this study, three different lipases derived from Lactobacillus brevis, Lactobacillus plantarum and their co-culture have been utilized to explore their efficiency towards PCL enzymatic degradation. The effect of parameters such as enzyme loading and degradation time has been explored to understand the efficiency of the enzymes used in this study. Various analysis, scanning electron microscopy and Fourier transform infrared spectroscopy have been employed to study the enzymatic degradation and its possible mechanistic insight.
通过脂肪酶实现的聚合物降解似乎是一种比传统化学方法更具吸引力的选择。聚(ε-己内酯)(PCL)是一种人造聚酯,可被微生物以及脂肪酶和酯酶等酶降解。PCL的环境降解取决于广泛存在于生态系统中的细菌活性,这些细菌可通过热重分析、差示热分析等表征技术进行研究。在本研究中,使用了源自短乳杆菌、植物乳杆菌的三种不同脂肪酶及其共培养物,以探索它们对PCL酶促降解的效率。研究了酶负载量和降解时间等参数的影响,以了解本研究中所用酶的效率。采用了各种分析方法、扫描电子显微镜和傅里叶变换红外光谱来研究酶促降解及其可能的作用机制。