菌株BIA产生的生物活性脂肽的纯化
Purification of Bioactive Lipopeptides Produced by Strain BIA.
作者信息
Alajlani Muaaz, Shiekh Abid, Hasnain Shahida, Brantner Adelheid
机构信息
Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4/I, 8010 Graz, Austria.
Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of the Punjab, Lahore, Pakistan.
出版信息
Chromatographia. 2016;79(21):1527-1532. doi: 10.1007/s10337-016-3164-3. Epub 2016 Sep 17.
strain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid-liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI-TOF-MS. The results revealed that strain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin.
菌株BIA用于生产生物活性脂肽。对不同的提取和纯化方法进行了测定，如液-液萃取、酸沉淀和硫酸铵沉淀，随后进行薄层层析（TLC）、固相萃取（SPE）和凝胶过滤。活性组分使用反相高效液相色谱（RP-HPLC）进一步纯化。通过Tris-三羟甲基氨基甲烷-十二烷基硫酸钠聚丙烯酰胺凝胶电泳（Tris-Tricine SDS-PAGE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）测定HPLC纯化产物的分子量。结果表明，菌株BIA产生了表面活性素和伊枯草菌素样化合物。该菌株同时产生表面活性素和伊枯草菌素样化合物，这对于一种潜在的生物防治剂来说是一个显著特征。本文还介绍了为从微生物来源中最佳且便捷地提取生物活性脂肽而开发的技术。
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