枯草芽孢杆菌 CMB32 生产脂肽类生物表面活性剂伊枯草菌素 A、芬枯草菌素和表面活性素 A 防治炭疽病菌。

Production of biosurfactant lipopeptides Iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides.

机构信息

Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea.

出版信息

J Microbiol Biotechnol. 2010 Jan;20(1):138-45.

PMID:20134245

Abstract

A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30degreesC. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reverse-phase HPLC. Molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1080, (b) 1486, and (c) 1044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, Thr, Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.

摘要

从土壤中分离出的一株具有潜在防治胶孢炭疽菌引起的炭疽病的细菌菌株被鉴定为枯草芽孢杆菌。枯草芽孢杆菌 CMB32 在 30°C 的 M9 肉汤中产生抗真菌剂。通过调节 pH 值至 2 并用氯仿/甲醇提取，枯草芽孢杆菌 CMB32 产生的生物表面活性剂脂肽被沉淀出来，然后通过柱层析和反相 HPLC 进行纯化。脂肽的分子量通过 MALDI-TOF 质谱法估计分别为（a）1080、（b）1486 和（c）1044 Da。它们具有环状结构和（a）Pro、Asx、Ser、Tyr、Glx、（b）Glx、Tyr、Thr、Ala、Pro、Ile 和（c）Glx、Leu、Val、Asx 的氨基酸组成。进一步分析表明，枯草芽孢杆菌 CMB32 产生了三种抗真菌脂肽：（a）伊枯草菌素 A、（b）丰原素和（c）表面活性素 A。