Noda-Nicolau Nathalia Mayumi, Polettini Jossimara, Peltier Morgan R, da Silva Márcia Guimarães, Menon Ramkumar
Division of Maternal-Fetal Medicine & Perinatal Research, Department of Obstetrics & Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, USA.
Department of Pathology, Botucatu Medical School, UNESP - Univ. Estadual Paulista, Botucatu, São Paulo, Brazil.
Am J Reprod Immunol. 2016 Dec;76(6):504-511. doi: 10.1111/aji.12596.
The polybacterial invasion and inflammation of the amniotic cavity is a common scenario in PTB, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections.
Fetal membranes were treated by heat-inactivated genital mycoplasmas and Gardnerella vaginalis at 10 or 10 colony/color-forming units/mL alone or in combination. Cytokines/receptors were measured in the medium by immunoassays.
Stimulation of genital mycoplasmas did not increase the proinflammatory cytokines, except Ureaplasma urealyticum that increased IL-8 levels. However, U. urealyticum and Mycoplasma hominis significantly increased IL-10 and IL-13 levels. G. vaginalis alone or in combination with genital mycoplasmas showed an increased proinflammatory and anti-inflammatory cytokines.
G. vaginalis sustain a proinflammatory response in the fetal membranes in vitro, while genital mycoplasmas induce a strong control of the inflammatory response. The ability of genital mycoplasmas to control the proinflammatory response may favor their survival in the upper genital tract.
羊膜腔的多菌性入侵和炎症是早产(PTB)中的常见情况,因此,我们分析了人胎膜的细胞因子产生情况,以更好地了解宿主对多菌性感染的反应。
用热灭活的生殖道支原体和阴道加德纳菌以10或10菌落/颜色形成单位/毫升单独或联合处理胎膜。通过免疫测定法测量培养基中的细胞因子/受体。
除解脲脲原体增加白细胞介素-8(IL-8)水平外,生殖道支原体的刺激并未增加促炎细胞因子。然而,解脲脲原体和人型支原体显著增加白细胞介素-10(IL-10)和白细胞介素-13(IL-13)水平。单独的阴道加德纳菌或与生殖道支原体联合使用时,促炎和抗炎细胞因子均增加。
阴道加德纳菌在体外胎膜中维持促炎反应,而生道支原体诱导对炎症反应的强力控制。生殖道支原体控制促炎反应的能力可能有利于它们在上生殖道中的存活。