Li Jiawei, Liu Tao, Huo Fangjun, Chao Jianbin, Zhang Yongbin, Yin Caixia
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Mar 5;174:17-24. doi: 10.1016/j.saa.2016.11.019. Epub 2016 Nov 14.
Two simple, efficient turn-on fluorescent probes for hypochlorite have been rationally designed and developed by utilizing the oxidation of hypochlorite. Notably, probe 1 and 2 displayed rapid and remarkable turn-on responses to ClO in PBS buffer solution (pH7.4). Further, the optical properties of two probes and their ClO-addition products were confirmed by density functional theory calculations. And detection limits of two probes for ClO based on the definition by IUPAC were calculated for 2.882nM and 0.354μM. More importantly, cell imaging experiments demonstrated that probe 1 was more suitable for detecting the ClO in living A549 cells. And both two probes had the possibility of potentially applied in practical applications such as detecting the hypochlorite concentration of tap water and river water.
通过利用次氯酸盐的氧化作用，合理设计并开发了两种用于检测次氯酸盐的简单、高效的开启型荧光探针。值得注意的是，探针1和2在PBS缓冲溶液（pH7.4）中对ClO表现出快速且显著的开启响应。此外，通过密度泛函理论计算证实了两种探针及其与ClO加成产物的光学性质。根据国际纯粹与应用化学联合会（IUPAC）的定义，计算出两种探针对ClO的检测限分别为2.882nM和0.354μM。更重要的是，细胞成像实验表明探针1更适合检测活的A549细胞中的ClO。并且这两种探针都有可能潜在地应用于实际应用中，如检测自来水和河水中的次氯酸盐浓度。
