College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
College of Life Sciences, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
Talanta. 2020 Aug 1;215:120892. doi: 10.1016/j.talanta.2020.120892. Epub 2020 Mar 2.
Highly sensitive specific and ultrasensitive fluorescent probes are employed for tracking ClO⁻ for revealing its various cellular functions in living cells. In this work, a near-infrared fluorescence probe, XS-1, has been developed for tracking ClO⁻. The experimental results demonstrated that the probe, XS-1, could determine ClO⁻ at the linear range of 0-2 μM with a low detection limit of 72 nM (λ = 580/626 nm). XS-1 can specifically detect ClO⁻, which can be applied for determining the basal ClO⁻ and the fluctuation of exogenous ClO⁻ in living cells. For further confirming its practicability, the probe was applied for detecting the hypochlorite content in the actual water sample. The recovery rate (92.7%-102.9%) displayed satisfactory values in the tap water and 84 disinfectants. In addition, we carried out theoretical calculations for clarifying the luminescence mechanism of the system.
高灵敏度、特异性和超灵敏荧光探针被用于跟踪 ClO⁻，以揭示其在活细胞中的各种细胞功能。在这项工作中，开发了一种近红外荧光探针 XS-1 用于跟踪 ClO⁻。实验结果表明，探针 XS-1 可以在 0-2 μM 的线性范围内确定 ClO⁻，检测限低至 72 nM（λ=580/626nm）。XS-1 可以特异性地检测 ClO⁻，可用于测定活细胞中的基础 ClO⁻和外源性 ClO⁻的波动。为了进一步确认其实用性，该探针被用于检测实际水样中的次氯酸盐含量。在自来水中和 84 种消毒剂中的回收率（92.7%-102.9%）显示出令人满意的值。此外，我们进行了理论计算，以阐明该体系的发光机制。
