妊娠肝内胆汁淤积症胎盘组织中实时逆转录聚合酶链反应数据标准化相关基因的鉴定
Identification of genes for normalization of real-time RT-PCR data in placental tissues from intrahepatic cholestasis of pregnancy.
作者信息
Li Yanli, Lu Huifang, Ji Yizhen, Wu Sufang, Yang Yongbin
机构信息
Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China.
Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China.
出版信息
Placenta. 2016 Dec;48:133-135. doi: 10.1016/j.placenta.2016.10.017. Epub 2016 Nov 2.
The selection of suitable reference genes is crucial for proper interpretation of RT-qPCR data. To date, a systematic screening for reference genes in placental tissues from intrahepatic cholestasis of pregnancy (ICP) is missing. Eighteen candidate reference genes were investigated to determine their applicability by descriptive statistics and published algorithms. HPRT1, YWHAZ and PUM1 were found to be the most stable genes and could be used for normalization in gene expression study of placentas from ICP. The combination of HPRT1 and YWHAZ is sufficient and the use of all three genes should be recommended for an improved reliability of normalization.
选择合适的内参基因对于正确解读RT-qPCR数据至关重要。迄今为止，尚缺乏对妊娠肝内胆汁淤积症（ICP）胎盘组织中的内参基因进行系统筛选。通过描述性统计和已发表的算法研究了18个候选内参基因，以确定它们的适用性。发现HPRT1、YWHAZ和PUM1是最稳定的基因，可用于ICP胎盘基因表达研究的标准化。HPRT1和YWHAZ的组合就足够了，建议使用所有三个基因以提高标准化的可靠性。
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