Soomro Razium Ali, Nafady Ayman, Hallam Keith Richard, Jawaid Sana, Al Enizi Abdullah, Sherazi Syed Tufail Hussain, Ibupoto Zafar Hussain, Willander Magnus
Interface Analysis Centre, School of Physics, University of Bristol, Bristol, BS8 1TL, UK; National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, 76080, Pakistan.
Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia; Department of Chemistry, Faculty of Science, Sohag University, Sohag, Egypt.
Anal Chim Acta. 2016 Dec 15;948:30-39. doi: 10.1016/j.aca.2016.11.015. Epub 2016 Nov 11.
This study describes sensitive determination of atropine using glassy carbon electrodes (GCE) modified with CoO nanostructures. The as-synthesised nanostructures were grown using cysteine (CYS), glutathione (GSH) and histidine (HYS) as effective templates under hydrothermal action. The obtained morphologies revealed interesting structural features, including both cavity-based and flower-shaped structures. The as-synthesised morphologies were noted to actively participate in electro-catalysis of atropine (AT) drug where GSH-assisted structures exhibited the best signal response in terms of current density and over-potential value. The study also discusses the influence of functional groups on the signal sensitivity of atropine electro-oxidation. The functionalisation was carried with the amino acids originally used as effective templates for the growth of CoO nanostructures. The highest increment was obtained when GSH was used as the surface functionalising agent. The GSH-functionalised CoO-modified electrode was utilised for the electro-chemical sensing of AT in a concentration range of 0.01-0.46 μM. The developed sensor exhibited excellent working linearity (R = 0.999) and signal sensitivity up to 0.001 μM of AT. The noted high sensitivity of the sensor is associated with the synergy of superb surface architectures and favourable interaction facilitating the electron transfer kinetics for the electro-catalytic oxidation of AT. Significantly, the developed sensor demonstrated excellent working capability when used for AT detection in human urine samples with strong anti-interference potential against common co-existing species, such as glucose, fructose, cysteine, uric acid, dopamine and ascorbic acid.
本研究描述了使用经CoO纳米结构修饰的玻碳电极（GCE）对阿托品进行灵敏测定。在水热作用下，以半胱氨酸（CYS）、谷胱甘肽（GSH）和组氨酸（HYS）作为有效的模板生长合成的纳米结构。所获得的形态呈现出有趣的结构特征，包括基于腔体的结构和花状结构。合成的形态被发现积极参与阿托品（AT）药物的电催化，其中GSH辅助的结构在电流密度和过电位值方面表现出最佳的信号响应。该研究还讨论了官能团对阿托品电氧化信号灵敏度的影响。官能化是用最初用作CoO纳米结构生长有效模板的氨基酸进行的。当使用GSH作为表面官能化剂时，获得了最高的增量。GSH官能化的CoO修饰电极用于浓度范围为0.01 - 0.46 μM的AT的电化学传感。所开发的传感器表现出出色的工作线性（R = 0.999）和高达0.001 μM AT的信号灵敏度。所观察到的传感器的高灵敏度与卓越的表面结构的协同作用以及促进AT电催化氧化的电子转移动力学的有利相互作用相关。值得注意的是，所开发的传感器在用于检测人尿液样本中的AT时表现出出色的工作能力，对常见的共存物质，如葡萄糖、果糖、半胱氨酸、尿酸、多巴胺和抗坏血酸具有很强的抗干扰潜力。
