利用高通量测序追踪抗生素耐药菌株的流行病学监测与分型方法
Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing.
作者信息
Machado Miguel Paulo, Ribeiro-Gonçalves Bruno, Silva Mickael, Ramirez Mário, Carriço João André
机构信息
Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal.
出版信息
Methods Mol Biol. 2017;1520:331-356. doi: 10.1007/978-1-4939-6634-9_20.
High-Throughput Sequencing (HTS) technologies transformed the microbial typing and molecular epidemiology field by providing the cost-effective ability for researchers to probe draft genomes, not only for epidemiological markers but also for antibiotic resistance and virulence determinants. In this chapter, we provide protocols for the analysis of HTS data for the determination of multilocus sequence typing (MLST) information and for determining presence or absence of antibiotic resistance genes.
高通量测序（HTS）技术改变了微生物分型和分子流行病学领域，它为研究人员提供了经济高效的能力来探测草图基因组，不仅用于流行病学标记，还用于抗生素耐药性和毒力决定因素。在本章中，我们提供了分析HTS数据的方案，用于确定多位点序列分型（MLST）信息以及确定抗生素耐药基因的存在与否。
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