Pereira R H V, Leão R S, Carvalho-Assef A P, Albano R M, Rodrigues E R A, Firmida M C, Folescu T W, Plotkowski M C, Bernardo V G, Marques E A
Departamento de Microbiologia,Imunologia e Parasitologia,Faculdade de Ciências Médicas,Universidade do Estado do Rio de Janeiro,Brazil.
Laboratório de Pesquisa em Infecção Hospitalar,Instituto Oswaldo Cruz,Rio de Janeiro,Brazil.
Epidemiol Infect. 2017 Feb;145(3):600-606. doi: 10.1017/S0950268816002624. Epub 2016 Nov 22.
Achromobacter spp. are opportunistic pathogens increasingly recovered from adult patients with cystic fibrosis (CF). We report the characterization of 122 Achromobacter spp. isolates recovered from 39 CF patients by multilocus sequence typing, virulence traits, and susceptibility to antimicrobials. Two species, A. xylosoxidans (77%) and A. ruhlandii (23%) were identified. All isolates showed a similar biofilm formation ability, and a positive swimming phenotype. By contrast, 4·3% and 44·4% of A. xylosoxidans and A. ruhlandii, respectively, exhibited a negative swarming phenotype, making the swimming and swarming abilities of A. xylosoxidans significantly higher than those of A. ruhlandii. A. xylosoxidans isolates from an outbreak clone also exhibited significantly higher motility. Both species were generally susceptible to ceftazidime, ciprofloxacin, imipenem and trimethoprim/sulphamethoxazole and there was no significant difference in susceptibility between isolates from chronic or sporadic infection. However, A. xylosoxidans isolates from chronic and sporadic cases were significantly more resistant to imipenem and ceftazidime than isolates of the outbreak clone.
无色杆菌属是越来越多地从成年囊性纤维化（CF）患者中分离出的机会致病菌。我们报告了通过多位点序列分型、毒力特征和对抗菌药物的敏感性，对从39例CF患者中分离出的122株无色杆菌属菌株进行的特征分析。鉴定出了两个种，即木糖氧化无色杆菌（77%）和鲁氏无色杆菌（23%）。所有分离株均表现出相似的生物膜形成能力和阳性的游动表型。相比之下，木糖氧化无色杆菌和鲁氏无色杆菌分别有4.3%和44.4%表现出阴性的群游表型，使得木糖氧化无色杆菌的游动和群游能力显著高于鲁氏无色杆菌。来自一个暴发克隆的木糖氧化无色杆菌分离株也表现出显著更高的运动性。两种菌通常对头孢他啶、环丙沙星、亚胺培南和甲氧苄啶/磺胺甲恶唑敏感，慢性或散发性感染的分离株之间在敏感性上没有显著差异。然而，慢性和散发性病例的木糖氧化无色杆菌分离株比暴发克隆的分离株对亚胺培南和头孢他啶的耐药性显著更高。
