Sharina Abu Hanifah, Lee Yook Heng, Musa Ahmad
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Sensors (Basel). 2008 Oct 16;8(10):6407-6416. doi: 10.3390/s8106407.
The role of incorporation of gold nanoparticles (50-130 nm in diameter) into a series of photocurable methacrylic-acrylic based biosensor membranes containing tyrosinase on the response for phenol detection was investigated. Membranes with different hydrophilicities were prepared from 2-hydroxyethyl methacrylate and n-butyl acrylate via direct photocuring. A range of gold nanoparticles concentrations from 0.01 to 0.5 % (w/w) was incorporated into these membranes during the photocuring process. The addition of gold nanoparticles to the biosensor membrane led to improvement in the response time by a reduction of approximately 5 folds to give response times of 5-10 s. The linear response range of the phenol biosensor was also extended from 24 to 90 mM of phenol. The hydrophilicities of the membrane matrices demonstrated strong influence on the biosensor response and appeared to control the effect of the gold nanoparticles. For less hydrophilic methacrylic-acrylic membranes, the addition of gold nanoparticles led to a poorer sensitivity and detection limit of the biosensor towards phenol. Therefore, for the application of gold nanoparticles in the enhancement of a phenol biosensor response, the nanoparticles should be immobilized in a hydrophilic matrix rather than a hydrophobic material.
研究了将直径为50 - 130纳米的金纳米颗粒掺入一系列含有酪氨酸酶的光固化甲基丙烯酸 - 丙烯酸酯基生物传感器膜中对苯酚检测响应的作用。通过直接光固化由甲基丙烯酸2 - 羟乙酯和丙烯酸正丁酯制备了具有不同亲水性的膜。在光固化过程中，将一系列浓度为0.01%至0.5%（w/w）的金纳米颗粒掺入这些膜中。向生物传感器膜中添加金纳米颗粒使响应时间缩短了约5倍，达到5 - 10秒，从而改善了响应时间。苯酚生物传感器的线性响应范围也从24毫摩尔扩展到了90毫摩尔的苯酚。膜基质的亲水性对生物传感器响应有很大影响，并且似乎控制着金纳米颗粒的作用。对于亲水性较差的甲基丙烯酸 - 丙烯酸酯膜，添加金纳米颗粒导致生物传感器对苯酚的灵敏度和检测限较差。因此，为了将金纳米颗粒应用于增强苯酚生物传感器的响应，纳米颗粒应固定在亲水性基质中，而不是疏水性材料中。
