Shan Dan, Zhu Mingjuan, Han En, Xue Huaiguo, Cosnier Serge
Key Laboratory of Environmental Materials & Environmental Engineering of Jiangsu Province, Yangzhou, China.
Biosens Bioelectron. 2007 Dec 15;23(5):648-54. doi: 10.1016/j.bios.2007.07.012. Epub 2007 Jul 31.
We reported on the utilization of a novel attractive nanoscaled calcium carbonate (nano-CaCO(3))-polyphenol oxidase (PPO) biocomposite to create a highly responsive phenol biosensor. The phenol sensor could be easily achieved by casting the biocomposite on the surface of glassy carbon electrode (GCE) via the cross-linking step by glutaraldehyde. The special three-dimensional structure, porous morphology, hydrophilic and biocompatible properties of the nano-CaCO(3) matrix resulted in high enzyme loading, and the enzyme entrapped in this matrix retained its activity to a large extent. The proposed PPO/nano-CaCO(3) exhibited dramatically developed analytical performance such as such as a broad determination range (6 x 10(-9) -2 x 10(-5)M), a short response time (less than 12 s), high sensitivity (474 mA M(-1)), subnanomolar detection limit (0.44 nM at a signal to noise ratio of 3) and good long-term stability (70% remained after 56 days). In addition, effects of pH value, applied potential, temperature and electrode construction were investigated and discussed.
我们报道了一种新型的具有吸引力的纳米碳酸钙（nano-CaCO₃）-多酚氧化酶（PPO）生物复合材料在构建高响应性酚类生物传感器中的应用。通过戊二醛交联步骤将生物复合材料浇铸在玻碳电极（GCE）表面，即可轻松制备酚类传感器。纳米CaCO₃基质特殊的三维结构、多孔形态、亲水性和生物相容性导致了高酶负载量，并且包裹在该基质中的酶在很大程度上保留了其活性。所提出的PPO/nano-CaCO₃表现出显著提升的分析性能，如宽测定范围（6×10⁻⁹ - ⁵2×10⁻⁵M）、短响应时间（小于12秒）、高灵敏度（474 mA M⁻¹）、亚纳摩尔检测限（在信噪比为3时为0.44 nM）以及良好的长期稳定性（56天后仍保留70%）。此外，还研究和讨论了pH值、施加电位、温度和电极结构的影响。
