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基于光固化聚丙烯酰胺膜的电化学日落黄生物传感器用于食品染料监测

Electrochemical Sunset Yellow Biosensor Based on Photocured Polyacrylamide Membrane for Food Dye Monitoring.

作者信息

Rozi Normazida, Ahmad Amalina, Yook Heng Lee, Shyuan Loh Kee, Hanifah Sharina Abu

机构信息

School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia.

Fuel Cell Institute, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia.

出版信息

Sensors (Basel). 2018 Jan 1;18(1):101. doi: 10.3390/s18010101.

DOI:10.3390/s18010101
PMID:29301262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5796456/
Abstract

An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide--ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide--ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0-101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.

摘要

研究了一种基于酶的电化学生物传感器用于分析日落黄合成食用色素。采用单步光聚合程序，在玻碳电极上涂覆聚（丙烯酰胺 - 甲基丙烯酸乙酯）膜以固定漆酶。聚（丙烯酰胺 - 甲基丙烯酸乙酯）膜表现出可接受的吸水性，适用于生物传感器应用。日落黄生物传感器的线性响应范围为0.08至10.00 μM，检测限为0.02 μM。该生物传感器成功用于测定软饮料中的日落黄，回收率为99.0 - 101.6％。使用高效液相色谱法对该方法进行了验证，表明该生物传感器可作为一种有前景的日落黄检测替代方法。

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