微小RNA-194通过靶向赖氨酸特异性去甲基化酶5B抑制胃癌细胞增殖和肿瘤发生。
miR-194 inhibits gastric cancer cell proliferation and tumorigenesis by targeting KDM5B.
作者信息
Bao J, Zou J-H, Li C-Y, Zheng G-Q
机构信息
Department of Nuclear Medicine, Department of Gastroenterology; Cangzhou Central Hospital, Cangzhou, Hebei, China.
出版信息
Eur Rev Med Pharmacol Sci. 2016 Nov;20(21):4487-4493.
OBJECTIVE
MicroRNAs play critical roles in regulating gene expression and various cellular processes in human cancer malignant progression. The aim of the present study was to examine the expression pattern of miR-194 in gastric cancer (GC) and its biological role in tumor progression.
MATERIALS AND METHODS
Using quantitative RT-PCR, we detected miR-194 expression in GC cell lines and primary tumor tissues. The proliferation, migration, and invasion assays were performed to investigate the effect of miR-194 on the GC cells. The target of miR-194 was predicted by TargetScan and confirmed by luciferase reporter assay. KDM5B expression was detected by Western blot.
RESULTS
miR-194 was significantly down-regulated in GC tissues and cell lines. Over-expression of miRNA-194 could inhibit GC cell proliferation, migration, and invasion in vitro. Also, miR-194 inhibited tumor growth and progression in vivo. Dual luciferase-based reporter assay indicated direct regulation of KDM5B by miR-194.
CONCLUSIONS
Our findings suggested that miR-194 directly targeted KDM5B and thereby acted as a tumor promoter in GC progression.
目的
微小RNA在调控人类癌症恶性进展中的基因表达和各种细胞过程中发挥关键作用。本研究的目的是检测miR-194在胃癌（GC）中的表达模式及其在肿瘤进展中的生物学作用。
材料与方法
使用定量逆转录聚合酶链反应，我们检测了GC细胞系和原发性肿瘤组织中miR-194的表达。进行增殖、迁移和侵袭实验以研究miR-194对GC细胞的影响。通过TargetScan预测miR-194的靶标，并通过荧光素酶报告基因检测进行验证。通过蛋白质免疫印迹法检测KDM5B的表达。
结果
miR-194在GC组织和细胞系中显著下调。miRNA-194的过表达可在体外抑制GC细胞的增殖、迁移和侵袭。此外，miR-194在体内抑制肿瘤生长和进展。基于双荧光素酶的报告基因检测表明miR-194直接调控KDM5B。
结论
我们的研究结果表明，miR-194直接靶向KDM5B，从而在GC进展中充当肿瘤促进因子。
Zotero 插件