Elieh-Ali-Komi Daniel, Kazemi Tohid, Shekari Najibeh, Farzamifar Parviz, Eghbali Elham, Mansoori Behzad, Baradaran Behzad, Shirmohamadi Masoud
Institute of Allergology, Charité, Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Allergology and Immunology, Berlin, Germany.
Iran J Public Health. 2024 Jul;53(7):1612-1620. doi: 10.18502/ijph.v53i7.16055.
Linoleic acid (LA) has modulatory effects on gastric cancer cell lines. This study aimed to investigate the effects of linoleic acid on the expression of metastatic and angiogenic molecular markers in gastric cancer cell line MKN-45.
In this study performed in Tabriz, Iran in 2021, MKN-45 cells were treated with LA in the presence or absence of docetaxel. Total RNA was extracted, and cDNA synthesized from the cells before and after treatment. The expression levels of Talin-2 and MMP-2 genes and mir-20, mir-30, mir-126, and mir-194, were determined by quantitative real-time PCR.
LA treatment reduced the expression levels of mir-126, mir-194, mir-30, and MMP-2, while increased the expression levels of Talin-2 mRNA. Docetaxel treatment could decrease the expression levels of mir-20, Talin-2, and MMP-2 mRNA levels while increasing the expression levels of mir-126, mir-194, and mir-30. Additionally, the combined treatment of MKN-45 cells with LA and docetaxel could reduce the expression levels of mir-20 and mir-126 and increased the expression levels of mir-194, mir-30, Talin-2, and MMP-2 mRNAs.
Modulation of the expression levels of gastric cancer involved microRNAs, Talin-2, and MMP-2 may be a mechanism through which LA may exert its biological effects on GC cell line MKN-45. LA may have an antimetastatic effect by reducing the MMP-2 expression and pro-angiogenic effect through increasing Talin-2 expression levels.
亚油酸（LA）对胃癌细胞系具有调节作用。本研究旨在探讨亚油酸对胃癌细胞系MKN-45中转移和血管生成分子标志物表达的影响。
2021年在伊朗大不里士进行的本研究中，在有或没有多西他赛的情况下用亚油酸处理MKN-45细胞。提取总RNA，并在处理前后从细胞中合成cDNA。通过定量实时PCR测定Talin-2和MMP-2基因以及mir-20、mir-30、mir-126和mir-194的表达水平。
亚油酸处理降低了mir-126、mir-194、mir-30和MMP-2的表达水平，同时增加了Talin-2 mRNA的表达水平。多西他赛处理可降低mir-20、Talin-2和MMP-2 mRNA水平的表达，同时增加mir-126、mir-194和mir-30的表达水平。此外，用亚油酸和多西他赛联合处理MKN-45细胞可降低mir-20和mir-126的表达水平，并增加mir-194、mir-30、Talin-2和MMP-2 mRNA的表达水平。
调节参与胃癌的微小RNA、Talin-2和MMP-2的表达水平可能是亚油酸对GC细胞系MKN-45发挥生物学作用的一种机制。亚油酸可能通过降低MMP-2表达具有抗转移作用，并通过增加Talin-2表达水平具有促血管生成作用。
