Bhattacharya Sourav, Das Arijit, Palaniswamy Muthusamy, Angayarkanni Jayaraman
Department of Microbiology, Center for Post Graduate Studies, Jain University, Bangalore, Karnataka, India.
Department of Microbiology, Karpagam University, Coimbatore, Tamil Nadu, India.
J Basic Microbiol. 2017 Feb;57(2):95-103. doi: 10.1002/jobm.201600479. Epub 2016 Nov 22.
Benzo[a]pyrene, a high molecular weight polycyclic aromatic hydrocarbon possesses carcinogenic, teratogenic, and mutagenic properties. The present study focuses on benzo[a]pyrene degradation by Pleurotus ostreatus PO-3, characterization and identification of metabolites produced and the extent of degradation in the presence of axenic culture of P. ostreatus PO-3 and defined co-cultures of the basidiomycete with bacteria and non-basidiomycete fungi. Thin-layer chromatography revealed that P. ostreatus PO-3 transformed benzo[a]pyrene to polar metabolites. Following degradation, appearance of numerous peaks in the mass spectrum indicated that benzo[a]pyrene degradation was a result of the metabolic activity of P. ostreatus PO-3. A degradation product corresponding to the m/z 284.2 was detected which could possibly be BaP-quinone, resulting from the oxidation of benzo[a]pyrene. Compared to the axenic culture of P. ostreatus PO-3 (64.3%), co-cultures of P. ostreatus PO-3 and Penicillium chrysogenum MTCC 787 and P. ostreatus PO-3 and Pseudomonas aeruginosa MTCC 1688 could degrade 86.1 and 75.1% of benzo[a]pyrene, respectively. Thus it could be inferred from the present investigation that the combined catabolic activities of P. ostreatus PO-3 with bacteria and non-basidiomycete fungi can produce synergistic effects to enhance BaP degradation. The increase in the generation of polar metabolites as degradation products from the recalcitrant parent compound advocates the potential application of P. ostreatus PO-3 in benzo[a]pyrene bioremediation.
苯并[a]芘是一种高分子量多环芳烃，具有致癌、致畸和致突变特性。本研究聚焦于平菇PO - 3对苯并[a]芘的降解、所产生代谢物的表征与鉴定，以及在平菇PO - 3无菌培养物存在的情况下，该担子菌与细菌及非担子菌真菌的特定共培养物中苯并[a]芘的降解程度。薄层色谱分析表明，平菇PO - 3将苯并[a]芘转化为极性代谢物。降解后，质谱图中出现众多峰，表明苯并[a]芘的降解是平菇PO - 3代谢活性的结果。检测到一个对应于质荷比284.2的降解产物，它可能是苯并[a]芘氧化产生的苯并[a]芘醌。与平菇PO - 3无菌培养物（64.3%）相比，平菇PO - 3与产黄青霉MTCC 787以及平菇PO - 3与铜绿假单胞菌MTCC 1688的共培养物分别能降解86.1%和75.1%的苯并[a]芘。因此，从本研究可以推断，平菇PO - 3与细菌及非担子菌真菌的联合分解代谢活动可产生协同效应，增强苯并[a]芘的降解。从难降解的母体化合物产生的极性代谢物作为降解产物的生成增加，表明平菇PO - 3在苯并[a]芘生物修复中具有潜在应用价值。
