Pozdnyakova N N, Chernyshova M P, Grinev V S, Landesman E O, Koroleva O V, Turkovskaya O V
Prikl Biokhim Mikrobiol. 2016 Nov-Dec;52(6):590-8.
The dependence of the degree of fluorene and fluoranthene degradation by the fungus Pleurotus ostreatus D1 on the culture medium composition has been studied. Polycyclic aromatic hydrocarbons (PAHs) have been transformed in Kirk’s medium (under conditions of laccase production) with the formation of a quinone metabolite and 9-fluorenone upon the use of fluoranthene and fluorene as substrates, respectively. More complete degradation with the formation of an intermediate metabolite, phthalic acid that has undergone subsequent utilization, has occurred in basidiomycete-rich medium (under the production of both laccase and versatile peroxidase). The formation of phthalic acid as a metabolite of fluoranthene degradation by lignolytic fungi has been revealed for the first time. The data allow the supposition that both extracellular laccase and laccase on the mycelium surface can participate in the initial stages of PAH metabolism, while versatile peroxidase is necessary for the oxidation of the formed metabolites. A scheme of fluorene metabolism by Pleurotus ostreatus D1 is suggested.
研究了平菇D1对芴和荧蒽的降解程度对培养基成分的依赖性。多环芳烃（PAHs）在Kirk培养基中（在漆酶产生的条件下）发生转化，以荧蒽和芴为底物时，分别形成醌类代谢产物和9-芴酮。在富含担子菌的培养基中（在漆酶和多功能过氧化物酶均产生的情况下），发生了更完全的降解，并形成了中间代谢产物邻苯二甲酸，随后该中间代谢产物被利用。首次揭示了木质素分解真菌将邻苯二甲酸作为荧蒽降解的代谢产物。这些数据表明，胞外漆酶和菌丝体表面的漆酶都可能参与多环芳烃代谢的初始阶段，而多功能过氧化物酶对于所形成代谢产物的氧化是必需的。提出了平菇D1对芴的代谢途径。
