Mouri T, Takahashi K, Sone M, Murakami O, Ohneda M, Itoi K, Imai Y, Yoshinaga K, Sasano N
Second Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
Peptides. 1989 Jan-Feb;10(1):201-4. doi: 10.1016/0196-9781(89)90097-1.
Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.
据报道，降钙素基因相关肽（CGRP）在甲状腺髓样癌的血浆和肿瘤组织中高浓度存在。采用放射免疫分析法研究了嗜铬细胞瘤肿瘤组织中的CGRP样免疫反应性（LI）。9例嗜铬细胞瘤中的CGRP-LI范围为0.50至1240 ng/g湿组织。葡聚糖G-50柱色谱显示，肿瘤提取物中的大多数CGRP-LI在与合成人CGRP相同的位置被洗脱。反相高压液相色谱显示，肿瘤提取物中的CGRP-LI在与合成人CGRP相同的位置以及更疏水的位置被洗脱。这些结果表明，嗜铬细胞瘤的肿瘤组织中也存在高浓度的CGRP-LI。
