Warwas M, Sawicki G
Department of Pharmaceutical Biochemistry, Medical Academy, Wrocław, Poland.
Acta Biochim Pol. 1989;36(1):7-15.
Two low-molecular protein fractions inhibiting cysteine proteases were isolated from human placenta by alkalization to pH 11, acetone fractionation, affinity chromatography on CM-papain-Sepharose 4B, and Sephadex G-75 gel filtration. The results of polyacrylamide gel electrophoresis, isoelectric focusing indicate that one of there fractions in a dimer of cystatins B, and another is a mixture of cystatins A and B.
通过碱化至pH 11、丙酮分级分离、在CM-木瓜蛋白酶-琼脂糖4B上进行亲和层析以及Sephadex G-75凝胶过滤，从人胎盘中分离出两种抑制半胱氨酸蛋白酶的低分子蛋白质组分。聚丙烯酰胺凝胶电泳和等电聚焦结果表明，其中一个组分是胱抑素B的二聚体，另一个是胱抑素A和B的混合物。
