Gureeva T A, Golubeva N V, Lubkova O N, Lokshina L A
Vopr Med Khim. 1994 May-Jun;40(3):6-8.
A procedure was developed for simultaneous isolation of aspartyl and cysteine proteinases as well as of the cysteine-proteinase inhibitors. Affinity chromatography using pepstatin-Sepharose enabled one to isolate aspartyl proteinases, while inhibitors of cysteine-proteinases were isolated by affinity chromatography on CM-papain-Sepharose; further purification of the enzymes was carried out using ion exchange chromatography and gel filtration. Partially purified preparations of cathepsin D as well as of cysteine-proteinases and their inhibitors were obtained. Some physicochemical and enzymatic properties of the enzymes and inhibitors obtained were studied.
已开发出一种同时分离天冬氨酸蛋白酶、半胱氨酸蛋白酶以及半胱氨酸蛋白酶抑制剂的方法。使用胃蛋白酶抑制剂-琼脂糖亲和层析可分离天冬氨酸蛋白酶，而通过CM-木瓜蛋白酶-琼脂糖亲和层析可分离半胱氨酸蛋白酶抑制剂；酶的进一步纯化则采用离子交换层析和凝胶过滤法。获得了组织蛋白酶D、半胱氨酸蛋白酶及其抑制剂的部分纯化制剂。对所获得的酶和抑制剂的一些理化及酶学性质进行了研究。
