犬和狼发生疫情后獾（欧洲獾）出现致命性犬瘟热。

Lethal distemper in badgers (Meles meles) following epidemic in dogs and wolves.

作者信息

Di Sabatino Daria, Di Francesco Gabriella, Zaccaria Guendalina, Malatesta Daniela, Brugnola Luca, Marcacci Maurilia, Portanti Ottavio, De Massis Fabrizio, Savini Giovanni, Teodori Liana, Ruggieri Enzo, Mangone Iolanda, Badagliacca Pietro, Lorusso Alessio

机构信息

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Campo Boario, 64100, Teramo, Italy.

Corpo Forestale dello Stato, Ufficio Territoriale per la Biodiversità, Viale Riviera 29, 65121, Pescara, Italy.

出版信息

Infect Genet Evol. 2016 Dec;46:130-137. doi: 10.1016/j.meegid.2016.10.020. Epub 2016 Oct 27.

DOI:10.1016/j.meegid.2016.10.020

PMID:27876612

Abstract

Canine distemper virus (CDV) represents an important conservation threat to many wild carnivores. A large distemper epidemic sustained by an Arctic-lineage strain occurred in Italy in 2013, mainly in the Abruzzi region, causing overt disease in domestic and shepherd dogs, Apennine wolves (Canis lupus) and other wild carnivores. Two badgers were collected by the end of September 2015 in a rural area of the Abruzzi region and were demonstrated to be CDV-positive by real time RT-PCR and IHC in several tissues. The genome of CDV isolates from badgers showed Y549H substitution in the mature H protein. By employing all publicly available Arctic-lineage H protein encoding gene sequences, six amino acid changes in recent Italian strains with respect to Italian strains of dogs from 2000 to 2008, were observed. A CDV strain belonging to the European-wildlife lineage was also identified in a fox found dead in the same region in 2016, proving co-circulation of an additional CDV lineage.

摘要

犬瘟热病毒（CDV）对许多野生食肉动物构成了重要的保护威胁。2013年，意大利发生了一场由北极谱系毒株引发的大规模犬瘟热疫情，主要集中在阿布鲁佐地区，导致家犬、牧羊犬、亚平宁狼（Canis lupus）和其他野生食肉动物出现明显病症。2015年9月底，在阿布鲁佐地区的一个农村地区采集到两只獾，通过实时RT-PCR和免疫组化在多个组织中证实它们为CDV阳性。来自獾的CDV分离株基因组在成熟H蛋白中显示出Y549H替换。通过使用所有公开可用的北极谱系H蛋白编码基因序列，观察到近期意大利毒株与2000年至2008年意大利犬类毒株相比有六个氨基酸变化。2016年，在同一地区发现一只死亡狐狸体内还鉴定出一种属于欧洲野生动物谱系的CDV毒株，证明还有另一种CDV谱系在共同传播。