Battista P J, Alila H W, Rexroad C E, Hansel W
Department of Physiology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Biol Reprod. 1989 Apr;40(4):769-75. doi: 10.1095/biolreprod40.4.769.
This study was conducted to characterize bovine platelets with respect to serotonin (5-HT) concentration and platelet-activating factor (PAF)-activation and to examine the in vitro effects of PAF and platelet-derived compounds on bovine luteal progesterone (P4) production. The concentration of 5-HT in platelets, as determined by high-performance liquid chromatography, was 538.8 +/- 40.83 ng/1 x 10(8) platelets. Based on a circulating platelet concentration range of 2.3 x 10(8) 5.8 x 10(8) platelets/ml, the circulating concentration of 5-HT would be approximately 1239-3125 ng/ml of blood. Bovine platelets were found to aggregate in response to PAF (1-40 ng/0.5 ml), with maximal aggregation occurring at 20-40 ng/0.5 ml. Coincubation of luteal cells with platelets (1 x 10(7)-4 x 10(8] enhanced luteal P4 production (p less than 0.05). Addition of the 5-HT receptor antagonist mianserin blocked the platelet-induced increases in P4 (p less than 0.05). Preincubation of platelets with indomethacin did not alter the production of P4 (p greater than 0.05), nor did the addition of propranolol (p greater than 0.05). Platelet-derived growth factor at 8 and 16 ng/ml enhanced basal P4 production (p less than 0.05) but had no effect on the responsiveness of luteal cells to luteinizing hormone (LH) (p greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在表征牛血小板中5-羟色胺（5-HT）浓度及血小板活化因子（PAF）活化情况，并检测PAF和血小板衍生化合物对牛黄体孕酮（P4）生成的体外影响。通过高效液相色谱法测定，血小板中5-HT浓度为538.8±40.83 ng/1×10⁸血小板。基于循环血小板浓度范围为2.3×10⁸-5.8×10⁸血小板/ml，5-HT的循环浓度约为1239-3125 ng/ml血液。发现牛血小板对PAF（1-40 ng/0.5 ml）有聚集反应，最大聚集发生在20-40 ng/0.5 ml。黄体细胞与血小板（1×10⁷-4×10⁸）共同孵育可增强黄体P4生成（p<0.05）。添加5-HT受体拮抗剂米安色林可阻断血小板诱导的P4增加（p<0.05）。血小板与吲哚美辛预孵育不改变P4生成（p>0.05），添加普萘洛尔也无影响（p>0.05）。8和16 ng/ml的血小板衍生生长因子可增强基础P4生成（p<0.05），但对黄体细胞对促黄体生成素（LH）的反应性无影响（p>0.05）。（摘要截短至250字）
