Battista P J, Condon W A
J Reprod Fertil. 1986 Jan;76(1):231-8. doi: 10.1530/jrf.0.0760231.
The addition of acetylcholine or histamine (10(-7) to 10(-4) M), gamma-aminobutyric acid, a dopamine agonist, and melatonin (10(-7) to 10(-5) M) did not alter basal or LH-stimulated progesterone production (P greater than 0.05). The addition of the specific beta 2-adrenergic agonist terbutaline and salbutamol did not significantly elevate progesterone production. Treatment of luteal cells with serotonin (5-HT), 10(-6) to 10(-4) M, increased the production of progesterone (P less than 0.05). This stimulated production was inhibited by the addition of mianserin (10(-5) M, a 5-HT antagonist; P less than 0.05). Isoproterenol (10(-7) to 10(-4) M) also resulted in significant increases in progesterone production (P less than 0.05). The combined treatments of 5-HT + LH, isoproterenol + LH, or isoproterenol + 5-HT did not result in a further increase in progesterone above that observed in response to LH or isoproterenol alone (P greater than 0.05). The isoproterenol-induced progesterone production could not be blocked by butoxamine (10(-5) M, a beta 2-antagonist), or practolol (10(-5) M, a beta 1-antagonist), but was inhibited by propranolol (10(-5) M, a general beta-antagonist; P less than 0.05). The response to isoproterenol was unaffected by mianserin (10(-5) M). These results demonstrate a possible role for 5-HT in the regulation of steroidogenesis by the corpus luteum of the cow. Furthermore, these results suggest that serotonin-induced progesterone production is a receptor-mediated event.
添加乙酰胆碱或组胺（10⁻⁷至10⁻⁴M）、γ-氨基丁酸、多巴胺激动剂和褪黑素（10⁻⁷至10⁻⁵M）不会改变基础或促黄体生成素刺激的孕酮生成（P>0.05）。添加特定的β₂-肾上腺素能激动剂特布他林和沙丁胺醇不会显著提高孕酮生成。用5-羟色胺（5-HT）（10⁻⁶至10⁻⁴M）处理黄体细胞会增加孕酮生成（P<0.05）。添加米安色林（10⁻⁵M，一种5-HT拮抗剂；P<0.05）可抑制这种刺激的生成。异丙肾上腺素（10⁻⁷至10⁻⁴M）也会导致孕酮生成显著增加（P<0.05）。5-HT + 促黄体生成素、异丙肾上腺素 + 促黄体生成素或异丙肾上腺素 + 5-HT的联合处理不会导致孕酮生成比单独对促黄体生成素或异丙肾上腺素的反应进一步增加（P>0.05）。异丙肾上腺素诱导的孕酮生成不能被丁氧胺（10⁻⁵M，一种β₂-拮抗剂）或心得宁（10⁻⁵M，一种β₁-拮抗剂）阻断，但被普萘洛尔（10⁻⁵M，一种通用的β-拮抗剂；P<0.05）抑制。对异丙肾上腺素的反应不受米安色林（10⁻⁵M）影响。这些结果证明了5-HT在牛黄体类固醇生成调节中的可能作用。此外，这些结果表明5-羟色胺诱导的孕酮生成是一个受体介导的事件。
