利用体外运动分析研究心肌肌球蛋白结合蛋白C对肌动蛋白-肌球蛋白相互作用中原肌球蛋白调节的影响。
Effect of Cardiac Myosin-Binding Protein C on Tropomyosin Regulation of Actin-Myosin Interaction Using In Vitro Motility Assay.
作者信息
Shchepkin D V, Kopylova G V, Nikitina L V
机构信息
Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
出版信息
Bull Exp Biol Med. 2016 Nov;162(1):45-47. doi: 10.1007/s10517-016-3541-9. Epub 2016 Nov 23.
We studied the modulating role of cardiac myosin-binding protein C (cMyBP-C) in tropomyosin regulation of the actin-myosin interaction. The effect of cMyBP-C on the velocity of actin-tropomyosin filament sliding over cardiac and slow skeletal myosins was evaluated using in vitro motility assay. The effect of cMyBP-C on the actin-tropomyosin filaments sliding depended on the type of myosin. The regulatory effect of cMyBP-C differs for cardiac and slow skeletal myosin because of the presence of specific essential light chain (LC1sa) in slow skeletal myosin isoform.
我们研究了心肌肌球蛋白结合蛋白C（cMyBP-C）在原肌球蛋白对肌动蛋白-肌球蛋白相互作用调节中的调控作用。使用体外运动分析评估了cMyBP-C对肌动蛋白-原肌球蛋白丝在心肌和慢骨骼肌肌球蛋白上滑动速度的影响。cMyBP-C对肌动蛋白-原肌球蛋白丝滑动的影响取决于肌球蛋白的类型。由于慢骨骼肌肌球蛋白异构体中存在特定的必需轻链（LC1sa），cMyBP-C对心肌和慢骨骼肌肌球蛋白的调节作用有所不同。
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