Zhang Yingyiing, Tang Bin, Du Guocheng
School of Biotechnology, Jiangnan University, Wuxi 214000, P.R. China.
College of Biochemical Engineering, Anhui Polytechnic University, Wuhu 241000, P.R. China.
J Microbiol Biotechnol. 2017 Mar 28;27(3):514-523. doi: 10.4014/jmb.1608.08048.
Carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in is still unclear. We extracted a carbon regulation gene, , that encoded a carbon catabolite repressor protein (CRE) from TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes ( and ) without . In the comparison of the response of and four cellulase genes to carboxymethylcellulose sodium and a simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars. By regulating the reducing sugars to range from 0.4% to 0.6%, a glucose-containing medium with lactose as the inducer could effectively induce cellulases without the repression of CRE. This regulation method could potentially reduce the cost of enzymes produced in industries and provide a possible solution to achieve the large-scale synthesis of cellulases.
碳分解代谢物阻遏是微生物中一种关键的调控机制，但其在[具体微生物名称未给出]中的特性仍不清楚。我们从[具体微生物名称未给出]TP - 02中提取了一个碳调控基因[基因名称未给出]，它编码一种碳分解代谢物阻遏蛋白（CRE），并通过实时定量PCR研究了CRE的调控作用。CRE在一定范围内对葡萄糖有反应，在没有[具体条件未给出]的情况下，它能显著调控部分纤维素酶基因（[基因名称未给出]和[基因名称未给出]）。在比较[具体物质未给出]和四个纤维素酶基因对羧甲基纤维素钠和一种简单碳源（乳糖）的反应时，CRE的作用仅与还原糖浓度有关。通过将还原糖调节至0.4%至0.6%的范围，以乳糖为诱导剂的含葡萄糖培养基可以有效诱导纤维素酶，而不会受到CRE的阻遏。这种调控方法有可能降低工业生产中酶的成本，并为实现纤维素酶的大规模合成提供一种可能的解决方案。
