Zhang Yingying, Tang Bin, Du Guocheng
School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
College of Biochemical Engineering, Anhui Polytechnic University, Wuhu, 241000, People's Republic of China.
Biotechnol Lett. 2018 Jun;40(6):973-979. doi: 10.1007/s10529-018-2549-9. Epub 2018 Apr 21.
To investigate the transcriptional regulation of cellobiose synthase (CBS) in Rhizopus stolonifer.
Transcription factor XYR1 was identified as responsible for the activation of cbs. In comparison with wild-type R. stolonifer, the deletion of XYR1 resulted in transcriptional down-regulation of cbs by approximately 40%, while XYR1 over-expression increased cbs transcription up to 175%. The highest FPA activity (1.8 IU/ml) was obtained in the XYR1-overexpressing strain OExyr1 cultivated in a 2% (m/V) glucose media, corresponding to a 96% increase compared with that of the parent strain (0.92 IU/ml). Moreover, cellulase synthesis was inhibited after cbs-inactivation mutation in OExyr1.
XYR1 directly activates the transcription of cbs to promote cellulase production in R. stolonifer utilizing glucose as a substrate.
研究匍枝根霉中纤维二糖合酶（CBS）的转录调控。
转录因子XYR1被确定为负责激活cbs。与野生型匍枝根霉相比，XYR1的缺失导致cbs转录下调约40%，而XYR1的过表达使cbs转录增加至175%。在2%（m/V）葡萄糖培养基中培养的XYR1过表达菌株OExyr1中获得了最高的FPA活性（1.8 IU/ml），与亲本菌株（0.92 IU/ml）相比增加了96%。此外，在OExyr1中进行cbs失活突变后，纤维素酶合成受到抑制。
XYR1直接激活cbs的转录，以促进匍枝根霉利用葡萄糖作为底物生产纤维素酶。
