通过下一代测序在男孩和女孩脐带血血沉棕黄层样本中差异表达的微小RNA。
miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls.
作者信息
Lizarraga Daneida, Huen Karen, Combs Mary, Escudero-Fung Maria, Eskenazi Brenda, Holland Nina
机构信息
School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
出版信息
Epigenomics. 2016 Dec;8(12):1619-1635. doi: 10.2217/epi-2016-0031. Epub 2016 Nov 24.
Abstract
AIM
Differences in children's development and susceptibility to diseases and exposures have been observed by sex, yet human studies of sex differences in miRNAs are limited.
MATERIALS & METHODS: The genome-wide miRNA expression was characterized by sequencing-based EdgeSeq assay in cord blood buffy coats from 89 newborns, and 564 miRNAs were further analyzed.
RESULTS
Differential expression of most miRNAs was higher in boys. Neurodevelopment, RNA metabolism and metabolic ontology terms were enriched among miRNA targets. The majority of upregulated miRNAs (86%) validated by nCounter maintained positive-fold change values; however, only 21% reached statistical significance by false discovery rate.
CONCLUSION
Accounting for host factors like sex may improve the sensitivity of epigenetic analyses for epidemiological studies in early childhood.
摘要
目的
已观察到儿童在发育以及对疾病和暴露的易感性方面存在性别差异，但关于微小RNA（miRNA）性别差异的人体研究有限。
材料与方法
采用基于测序的EdgeSeq分析方法对89例新生儿脐带血 Buffy 层进行全基因组miRNA表达特征分析，并对564个miRNA进行进一步分析。
结果
大多数miRNA在男孩中的差异表达更高。miRNA靶标中神经发育、RNA代谢和代谢本体术语富集。通过nCounter验证的大多数上调miRNA（86%）保持正倍数变化值；然而，按错误发现率计算，只有21%达到统计学显著性。
结论
考虑性别等宿主因素可能会提高儿童早期流行病学研究中表观遗传学分析的敏感性。
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